Tanshinones from Salvia miltiorrhiza inhibit Mycobacterium tuberculosis via disruption of the cell envelope surface and oxidative stress
| Autor: | Rafal Sawicki, Wieslaw Truszkiewicz, Elwira Sieniawska, Andrey S. Marchev, Milen I. Georgiev |
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| Rok vydání: | 2021 |
| Předmět: |
Cell envelope
Cell Mycobactin Salvia miltiorrhiza Microbial Sensitivity Tests Toxicology medicine.disease_cause Plant Roots Bacterial cell structure Mass Spectrometry 03 medical and health sciences medicine Metabolomics Lipidomics Transcriptomics Sigma factors LC-MS Chromatography High Pressure Liquid 030304 developmental biology 0303 health sciences 030306 microbiology Chemistry Plant Extracts General Medicine Mycobacterium tuberculosis Lipids 3. Good health Cell biology Anti-Bacterial Agents Metabolic pathway Oxidative Stress medicine.anatomical_structure Abietanes Bacterial outer membrane Oxidative stress Food Science |
| Zdroj: | Food and Chemical Toxicology |
| ISSN: | 1873-6351 |
| Popis: | The unique structure of Mycobacterium tuberculosis cell envelope provides impermeable barrier against environmental stimuli. In the situation that this barrier is disturbed Mycobacteria react at the transcriptional and translational level to redirect metabolic processes and to maintain integrity of the cell. In this work we aimed to explore the early metabolic response of M. tuberculosis to tanshinones, which are active antimycobacterial compounds of Salvia miltiorrhiza Bunge root. The investigation of the expression of sigma factors revealed the significant shifts in the general bacterial regulatory network, whereas LC-MS metabolomics evidenced the changes in the composition of bacterial cell envelope and indicated altered metabolic pathways. Tanshinones acted via the disruption of the cell envelope surface and generation of reactive oxygen species. Bacteria responded with overproduction of inner region of outer membrane, fluctuations in the production of glycerophosphoinositolglycans, as well as changes in the levels of mycobactins, accompanied by enrichment of metabolic pathways related to redox balance and repair of damages caused by tanshinones. |
| Databáze: | OpenAIRE |
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