Non-targeted Metabolomic Analysis Based on Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry Reveals the Effects of Grafting on Non-volatile Metabolites in Fresh Tea Leaves (Camellia sinensis L.)
| Autor: | Qi Dandan, Qiao Xiaoyan, Guo Weiqing, Aiqing Miao, Chengying Ma, Meiling Lu, Chen Wei, Junxing Li |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Tandem mass spectrometry 01 natural sciences Camellia sinensis chemistry.chemical_compound Metabolomics Biosynthesis Tandem Mass Spectrometry Volatile metabolites Chromatography High Pressure Liquid Plant Proteins Chromatography Plant Extracts Chemistry 010401 analytical chemistry General Chemistry Grafting 0104 chemical sciences Plant Leaves Plant Breeding Flavonoid biosynthesis General Agricultural and Biological Sciences Rootstock 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:6672-6682 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b01001 |
| Popis: | To investigate the effects of grafting on non-volatile metabolites in tea, non-targeted metabolomic analyses of fresh leaves were performed on the basis of ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF/MS). One non-grafted YingHong No. 9 and four grafted tea [grafting scion YingHong No. 9 on four different rootstocks, BaiMao No. 2 (BM2), BaiYeDanCong (BY), HeiYeShuiXian (HY), and WuLingHong (WLH)] were chosen as materials. In total, 32 differential metabolites were identified, including phenolic acids, flavan-3-ols, dimeric catechins, flavonol and flavonol/flavone glycosides, etc. Partial least squares discrimination analysis and hierarchical cluster analysis showed various effects of different rootstocks on metabolites. Thereinto, rootstocks of WLH and BY showed extremely outstanding performance in up- and downregulating these metabolites, respectively. Differential metabolites were enriched into three crucial pathways, including biosynthesis of phenylpropanoids, flavonoid biosynthesis, and flavone and flavonol biosynthesis, which might influence the quality of tea. This study provides a theoretical basis for grafting-related variations of non-volatile metabolites in fresh tea leaves. |
| Databáze: | OpenAIRE |
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