Changing expression profiles of mRNA, lncRNA, circRNA, and miRNA in lung tissue reveal the pathophysiological of bronchopulmonary dysplasia (BPD) in mouse model
| Autor: | Xiangyun Yan, Yin Hu, Jing Yin, Xingyun Wang, Juan Wang, Heng Liu, Shuping Han, Bin Zhuang, Zhangbin Yu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biology Bioinformatics behavioral disciplines and activities Biochemistry Pathogenesis Mice 03 medical and health sciences 0302 clinical medicine mental disorders microRNA medicine Animals Humans RNA Messenger KEGG Lung Molecular Biology Bronchopulmonary Dysplasia Messenger RNA Sequence Analysis RNA Gene Expression Profiling High-Throughput Nucleotide Sequencing RNA Circular Cell Biology medicine.disease Pathophysiology Disease Models Animal MicroRNAs Gene Ontology 030104 developmental biology Gene Expression Regulation Bronchopulmonary dysplasia 030220 oncology & carcinogenesis RNA Long Noncoding Lung tissue Function (biology) |
| Zdroj: | Journal of Cellular Biochemistry. 120:9369-9380 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | New perinatal care technologies have improved the survival rate of preterm neonates, but the prevalence of bronchopulmonary dysplasia (BPD), one of the most intractable problems in neonatal intensive care unit (NICU), remains unchanged. In present study, high-throughput sequencing (HTS) was performed to detect the expression profiles of long noncoding RNAs (lncRNAs), messenger RNAs (mRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) in hyperoxia-induced BPD mouse model. Significant differentially expressed RNAs were selected and clustered between the BPD group and the control group. The results revealed that expressions of 1778 lncRNAs, 1240 mRNAs, 97 circRNAs, and 201 miRNAs were significantly altered in the BPD group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed to predict the potential functions of differentially expressed RNAs. lncRNA-mRNA and circRNA-miRNA coexpression networks were constructed to detect their association with the pathogenesis of BPD. Our study provides a systematic perspective on the potential function of RNAs during BPD. |
| Databáze: | OpenAIRE |
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