Protein phosphatase 1 β paralogs encode the zebrafish myosin phosphatase catalytic subunit
| Autor: | Joshua B. Rosales, Brandon W. Carr, Michael J. Nguyen, Douglas C. Weiser, Joseph B. Rosales, Vaishali Jayashankar, Dale C. Zheng |
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| Rok vydání: | 2013 |
| Předmět: |
Myosin light-chain kinase
Myosin Light Chains Science Phosphatase Molecular Sequence Data macromolecular substances Biology 03 medical and health sciences Mice Myosin-Light-Chain Phosphatase 0302 clinical medicine Catalytic Domain Protein Phosphatase 1 Sequence Homology Nucleic Acid Myosin Animals Humans Amino Acid Sequence Phosphorylation Zebrafish 030304 developmental biology 0303 health sciences Multidisciplinary Gene Expression Regulation Developmental Protein phosphatase 1 Protein phosphatase 2 Actin cytoskeleton Actin Cytoskeleton Myosin phosphatase complex HEK293 Cells Biochemistry Medicine Myosin-light-chain phosphatase Cardiac Myosins 030217 neurology & neurosurgery HeLa Cells Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 9, p e75766 (2013) |
| ISSN: | 1932-6203 |
| Popis: | BackgroundThe myosin phosphatase is a highly conserved regulator of actomyosin contractility. Zebrafish has emerged as an ideal model system to study the in vivo role of myosin phosphatase in controlling cell contractility, cell movement and epithelial biology. Most work in zebrafish has focused on the regulatory subunit of the myosin phosphatase called Mypt1. In this work, we examined the critical role of Protein Phosphatase 1, PP1, the catalytic subunit of the myosin phosphatase.Methodology/principal findingsWe observed that in zebrafish two paralogous genes encoding PP1β, called ppp1cba and ppp1cbb, are both broadly expressed during early development. Furthermore, we found that both gene products interact with Mypt1 and assemble an active myosin phosphatase complex. In addition, expression of this complex results in dephosphorylation of the myosin regulatory light chain and large scale rearrangements of the actin cytoskeleton. Morpholino knock-down of ppp1cba and ppp1cbb results in severe defects in morphogenetic cell movements during gastrulation through loss of myosin phosphatase function.Conclusions/significanceOur work demonstrates that zebrafish have two genes encoding PP1β, both of which can interact with Mypt1 and assemble an active myosin phosphatase. In addition, both genes are required for convergence and extension during gastrulation and correct dosage of the protein products is required. |
| Databáze: | OpenAIRE |
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