Effects of androgen on 17.BETA.-estradiol production by cultured human granulosa cells
| Autor: | Akira Yajima, Akira Tsuiki, Shinji Satoh, Osamu Shinkawa, Takao Fukaya, Yuichi Wada, Hiroshi Hoshiai, Masayuki Horiguchi |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty Time Factors medicine.drug_class media_common.quotation_subject Cellular differentiation In Vitro Techniques General Biochemistry Genetics and Molecular Biology Internal medicine medicine Humans Aromatase Cells Cultured Testosterone media_common Granulosa Cells Estradiol biology urogenital system Chemistry Dihydrotestosterone General Medicine Luteinizing Hormone Androgen In vitro Androgen receptor Endocrinology biology.protein Female medicine.drug |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 154:253-260 |
| ISSN: | 1349-3329 0040-8727 |
| DOI: | 10.1620/tjem.154.253 |
| Popis: | In order to determine whether androgen acts solely as a substrate for aromatization or whether it also influences on the activity of aromatase enzyme, human granulosa cells were incubated in vitro with or without androgen. Although basal production of 17 beta-estradiol (E2) in cultured granulosa cells obtained from follicles about 26 hr after the initiation of the LH surge was restricted in small quantities, a marked increase in E2 production occurred in the presence of testosterone (T) (10(-6) M) as aromatizable substrate. The non-aromatizable androgen, 5 alpha-dihydrotestosterone (DHT) (10(-7) M, 10(-6) M), slightly enhanced E2 production and it did not inhibit T aromatization in these cells. By contrast, DHT did not increase E2 production in granulosa cells obtained from follicles 2-5 days before the LH surge. The results indicate that androgen can enhance the estrogen biosynthesis of granulosa cells obtained from follicles during the LH surge not only by acting as a substrate for aromatization but also by participating in some process of the estrogen synthesis. This effect of androgen was not clearly seen in granulosa cells obtained before the LH surge, suggesting that the response of granulosa cells to exogenous androgen varies with the stage of the cell differentiation. |
| Databáze: | OpenAIRE |
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