The concentration of antibiotic in fresh-frozen bone graft
| Autor: | Hsin-Nung Shih, D. W. Chen, Steve Wn Ueng, Mel S. Lee, Pang-Hsin Hsieh, Yuhan Chang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Staphylococcus aureus medicine.medical_specialty Time Factors food.ingredient medicine.drug_class Arthroplasty Replacement Hip medicine.medical_treatment Antibiotics Cefazolin Osteoarthritis Osteoarthritis Hip Femoral head food Disk Diffusion Antimicrobial Tests Living Donors medicine Humans Transplantation Homologous Agar Orthopedics and Sports Medicine Femur Chromatography High Pressure Liquid Aged Antibacterial agent Aged 80 and over Cryopreservation business.industry Femur Head Organ Preservation Middle Aged medicine.disease Arthroplasty Anti-Bacterial Agents Femoral Neck Fractures Surgery medicine.anatomical_structure Anesthesia Injections Intravenous Tissue and Organ Harvesting Female business medicine.drug |
| Zdroj: | The Journal of Bone and Joint Surgery. British volume. :1471-1474 |
| ISSN: | 2044-5377 0301-620X |
| DOI: | 10.1302/0301-620x.92b10.24704 |
| Popis: | We investigated the antibiotic concentration in fresh-frozen femoral head allografts harvested from two groups of living donors. Ten samples were collected from patients with osteoarthritis of the hip and ten from those with a fracture of the neck of the femur scheduled for primary arthroplasty. Cefazolin (1 g) was administered as a pre-operative prophylactic antibiotic. After storage at −80°C for two weeks the pattern of release of cefazolin from morsellised femoral heads was evaluated by an in vitro broth elution assay using high-performance liquid chromatography. The bioactivity of the bone was further determined with an agar disc diffusion and standardised tube dilution bioassay. The results indicated that the fresh-frozen femoral heads contained cefazolin. The morsellised bone released cefazolin for up to four days. The concentration of cefazolin was significantly higher in the heads from patients with osteoarthritis of the hip than in those with a fracture. Also, in bioassays the bone showed inhibitory effects against bacteria. We concluded that allografts of morsellised bone from the femoral head harvested from patients undergoing arthroplasty of the hip contained cefazolin, which had been administered pre-operatively and they exhibited inhibitory effects against bacteria in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|