Importance of Methodology in Determination of Chlamydia pneumoniae Seropositivity in Healthy Subjects and in Patients with Coronary Atherosclerosis
| Autor: | C.J. Vrints, Margareta Ieven, L. Van Renterghem, D. Ursi, Vicky Y. Hoymans, Johan Bosmans, Rudolf P. Mak, Floris L. Wuyts |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Microbiology (medical) Chlamydiology and Rickettsiology Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Coronary Artery Disease medicine.disease_cause Immunoglobulin G Serology Immunoenzyme Techniques Risk Factors Seroepidemiologic Studies medicine Humans Seroprevalence Chlamydiaceae Chlamydia biology business.industry Chlamydophila pneumoniae Middle Aged biology.organism_classification medicine.disease Antibodies Bacterial Chlamydiales Immunology biology.protein Antibody business |
| Zdroj: | Journal of clinical microbiology |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Enzyme immunoassays (EIAs) for the detection of Chlamydia pneumoniae antibodies were compared to the microimmunofluorescence (MIF) test, the reference method. Furthermore, we assessed the hypothesis that a possible relationship between Chlamydia pneumoniae immunoglobulin G (IgG) antibodies and coronary artery disease is dependent on the type of EIA. Sera from 112 healthy men (mean age, 50.1 years) were tested for antibodies against Chlamydia pneumoniae by five commercial test kits: Focus Chlamydia MIF IgG test, Labsystems Chlamydia pneumoniae IgG EIA (LS EIA), R-Biopharm Elegance Chlamydia pneumoniae IgG EIA (RB EIA), Medac Chlamydia pneumoniae IgG sandwich enzyme-linked immunosorbent assay ELISA (MCp sELISA) and Medac Chlamydia IgG recombinant enzyme-linked immunosorbent assay ELISA (MC rELISA). Sera from 106 consecutive male patients (mean age, 63.6 years) undergoing diagnostic coronary angiography were also examined using the Focus MIF, LS EIA, MCp sELISA, and MC rELISA techniques. The agreement between LS EIA (65 to 83% [controls-patients]) or MC rELISA (49 to 61%) and Focus MIF (78 to 83%) was average to fair (κ = 0.597 and 0.234, respectively). MCp sELISA and RB EIA showed good agreement with MIF (κ = 0.686 and 0.665, respectively), with 80 to 89 and 79% of individuals reacting positively. A significant difference in seroprevalence between patients and healthy subjects was observed with the LS EIA, while seropositivities in the two study groups appeared equal when the Focus MIF assay was applied. The MC rELISA and MCp sELISA gave statistically significant differences in antibody seroprevalence in patients with two-vessel disease or when the patient group combined individuals with a two- or a three-vessel disease, respectively. The concordance between MIF and other commonly used serological assays for C. pneumoniae IgG antibody detection is good to fair. The choice of serological assay has important implications for C. pneumoniae antibody seroprevalence, as well as for the relationship between C. pneumoniae seropositivity and coronary artery disease. |
| Databáze: | OpenAIRE |
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