An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance
| Autor: | Ha Thi Kim Nguyen, Hae Jin Kim, Sang Dong Yoo, Sujin Hyoung, Yun Young Kim, Jeong Sheop Shin, Mei Hua Cui, Kyoung Shin Yoo, Sung Han Ok |
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| Rok vydání: | 2013 |
| Předmět: |
Salt stress
Biophysics Arabidopsis Intracellular Space Down-Regulation Biochemistry Serine chemistry.chemical_compound Structural Biology Stress Physiological Genetics Phosphoprotein Phosphatases Arabidopsis thaliana Electrophoretic mobility shift assay MYB Threonine Promoter Regions Genetic Molecular Biology Abscisic acid Transcription factor biology Arabidopsis Proteins fungi food and beverages Cell Biology Salt Tolerance biology.organism_classification Plants Genetically Modified Adaptation Physiological Protein Phosphatase 2C Protein Transport chemistry Seedlings Type 2C serine/threonine protein phosphatase MYB family Abscisic Acid Signal Transduction Transcription Factors |
| Zdroj: | FEBS letters. 587(12) |
| ISSN: | 1873-3468 |
| Popis: | We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance. |
| Databáze: | OpenAIRE |
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