Granulokinetics in normal dogs
| Autor: | D. R. Boggs, J. W. Athens, George E. Cartwright, Maxwell M. Wintrobe, S. O. Raab, O. P. Haab, H. Ashenbrucker |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
medicine.medical_specialty
Pentobarbital Isoflurophate Epinephrine Granulocyte chemistry.chemical_compound Blood Circulation Time Dogs Physiology (medical) Internal medicine Leukocytes medicine Whole blood Pharmacology Total blood Hexadimethrine bromide Chemistry Research Phosphorus Isotopes In vitro medicine.anatomical_structure Endocrinology Splenectomy medicine.drug |
| Zdroj: | American Journal of Physiology-Legacy Content. 206:83-88 |
| ISSN: | 0002-9513 |
| DOI: | 10.1152/ajplegacy.1964.206.1.83 |
| Popis: | Dog granulocytes were labeled in vitro with radioactive diisopropylfluorophosphate (DFP32) and then returned to the circulation of the donor. Granulocytes were separated from whole blood by utilizing hexadimethrine bromide as the sedimenting agent and saponin as a lysing agent. The labeled granulocytes disappeared from the circulation in an exponential fashion with a mean (±1 sd) half-time disappearance of 5.6 ± 0.95 hr. The size of the total blood granulocyte ( TBGP), circulating granulocyte ( CGP), and marginal granulocyte ( MGP) pools, and the granulocyte turnover rate ( GTR) were measured in 31 normal, unanesthetized dogs. The mean values ± 1 sd, expressed as number of cells x107/kg body wt., were as follows: TBGP, 102 ± 34.8; CGP, 54 ± 20.7; MGP, 48 ± 23.4; and GTR, 305 ± 111.5 cells/kg day. The values observed in anesthetized and in unanesthetized, splenectomized dogs were not significantly different from the above values. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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