IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage

Autor: P. Behrendt, A. Preusse-Prange, Bernd Rolauffs, T. Klüter, M. Haake, Alan J. Grodzinsky, Bodo Kurz, S. Lippross
Rok vydání: 2016
Předmět:
Zdroj: Osteoarthritis and Cartilage. 24:1981-1988
ISSN: 1063-4584
DOI: 10.1016/j.joca.2016.06.016
Popis: Summary Objective The aim of this study was to examine whether anti-inflammatory interleukin-10 (IL-10) exerts chondroprotective effects in an in vitro model of a single mechanical injury of mature articular cartilage. Method Articular cartilage was harvested from the femoro-patellar groove of adult cows ( Bos taurus ) and cultured w/o bovine IL-10. After 24 h of equilibration explants were subjected to an axial unconfined compression (50% strain, velocity 2 mm/s, held for 10 s). After 96 h cell death was measured histomorphometrically (nuclear blebbing, NB) and the release of glycosaminoglycans (GAG, DMMB assay) and nitric oxide (NO, Griess-reagent) were analyzed. mRNA levels of matrix degrading enzymes and nitric oxide synthetase were measured by quantitative real time PCR. Differences between groups were calculated using a one-way ANOVA with a Bonferroni post hoc test. Results Injurious compression significantly increased the number of cells with NB, release of GAG and nitric oxide and expression of MMP-3, -13, ADAMTS-4 and NOS2. Administration of IL-10 significantly reduced the injury related cell death and release of GAG and NO, respectively. Expression of MMP-3, -13, ADAMTS-4 and NOS2 were significantly reduced. Conclusion Joint injury is a complex process involving specific mechanical effects on cartilage as well as induction of an inflammatory environment. IL-10 prevented crucial mechanisms of chondrodegeneration induced by an injurious single compression. IL-10 might be a multipurpose drug candidate for the treatment of cartilage-related sports injuries or osteoarthritis (OA).
Databáze: OpenAIRE
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