Exploring the effective materials of flavonoids-enriched extract from Scutellaria baicalensis roots based on the metabolic activation in influenza A virus induced acute lung injury
| Autor: | Yun-Yi Zhang, Xin Jin, Yan Lu, Zhu Haiyan, Hong Li, Zhi Haijuan, Daofeng Chen |
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| Rok vydání: | 2019 |
| Předmět: |
Clinical Biochemistry
Acute Lung Injury Pharmaceutical Science Pharmacology Lung injury medicine.disease_cause 01 natural sciences Plant Roots Analytical Chemistry Activation Metabolic chemistry.chemical_compound Mice Wogonin In vivo Drug Discovery Influenza Human Influenza A virus medicine Animals Humans Chrysin Lung Spectroscopy Glucuronidase Flavonoids biology 010405 organic chemistry 010401 analytical chemistry biology.organism_classification 0104 chemical sciences Baicalein Gastrointestinal Microbiome Specific Pathogen-Free Organisms Disease Models Animal Complement Inactivating Agents chemistry Scutellaria baicalensis Oroxylin A Drugs Chinese Herbal |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 177 |
| ISSN: | 1873-264X |
| Popis: | Flavonoids-enriched extract from Scutellaria baicalensis roots (FESR) ameliorated influenza A virus (IAV) induced acute lung injury (ALI) in mice by inhibiting the excessive activation of complement system in vivo. However, FESR had no anti-complementary activity in vitro. In order to reveal the effective materials of FESR for the treatment of IAV-induced ALI, the present research explored the metabolic process of FESR both in nomal and IAV infected mice by the method of UHPLC-ESI-LTQ/MS, as well as the metabolic activating mechanism. The results showed that the inactive flavonoid glycosides of FESR were partly metabolized into anti-complementary aglycones in vivo, mainly including 5,7,4'-trihydroxy-8-methoxy-flavone, norwogonin, baicalein, wogonin, oroxylin A and chrysin. Moreover, compared with the normal mice, IAV-induced ALI mice exhibited more efficient on producing and absorbing these active metabolites, with AUC0-t and Cmax in plasma and concentrations in lungs and intestines markedly elevated in the IAV treated groups (P |
| Databáze: | OpenAIRE |
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