Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

Autor:	Emilio I. Alarcon, Olga García, Andrea Rivas-Aravena, Sabrina Simoncelli, Alexis Aspée, Marta Liras, J. C. Scaiano
Rok vydání:	2016
Předmět:	Boron Compounds Nitroxide mediated radical polymerization 010402 general chemistry Photochemistry 01 natural sciences Biochemistry chemistry.chemical_compound Hydroxylamine Amide Fluorescence microscope Humans Physical and Theoretical Chemistry Hydrogen peroxide Cells Cultured Fluorescent Dyes Total internal reflection fluorescence microscope 010405 organic chemistry Organic Chemistry Hydrogen Peroxide Fibroblasts Fluorescence Amides 0104 chemical sciences chemistry Microscopy Fluorescence Nitrogen Oxides BODIPY Oxidation-Reduction
Zdroj:	Organicbiomolecular chemistry. 14(17)
ISSN:	1477-0539
Popis:	A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eace44a528732c5c734e576bd686cec https://pubmed.ncbi.nlm.nih.gov/27065020 Zobrazit plný text záznamu