Patterning of mammalian somites by surface ectoderm and notochord: Evidence for sclerotome induction by a hedgehog homolog
| Autor: | Chen-Ming Fan, Marc Tessier-Lavigne |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
animal structures
Molecular Sequence Data Notochord Dermomyotome General Biochemistry Genetics and Molecular Biology Cell Line Mesoderm Mice Culture Techniques Ectoderm medicine Paraxial mesoderm Animals Hedgehog Proteins Sonic hedgehog Hedgehog Floor plate Embryonic Induction Base Sequence biology Neural tube Proteins Anatomy Surface ectoderm Cell biology medicine.anatomical_structure embryonic structures Trans-Activators biology.protein Signal Transduction |
| Zdroj: | Cell. 79:1175-1186 |
| ISSN: | 0092-8674 |
| Popis: | An early step in the development of vertebrae, ribs, muscle, and dermis is the differentiation of the somitic mesoderm into dermomyotome dorsally and sclerotome ventrally. To analyze this process, we have developed an in vitro assay for somitic mesoderm differentiation. We show that sclerotomal markers can be induced by a diffusible factor secreted by notochord and floor plate and that heterologous cells expressing Sonic hedgehog (shh/vhh-1) mimic this effect. In contrast, expression of dermomyotomal markers can be caused by a contact-dependent signal from surface ectoderm and a diffusible signal from dorsal neural tube. Our results extend previous studies by suggesting that dorsoventral patterning of somites involves the coordinate action of multiple dorsalizing and ventralizing signals and that a diffusible form of Shh/Vhh-1 mediates sclerotome induction. |
| Databáze: | OpenAIRE |
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