Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells
| Autor: | M. Cristina Kenney, Saffar Mansoor, Baruch D. Kuppermann, Sonali Nashine, N. Gupta, Ashish U. Sapkal, Rafael Migon, Vishal R. Sharma, Claudio Ramirez, Marilyn Chwa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Programmed cell death Retinal Pigment Epithelial Cells Apoptosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Ophthalmology Opthalmology and Optometry beta-Estradiol Medicine Viability assay Membrane potential business.industry Smoking Benzo(e)pyrene Molecular biology 17β-Estradiol Ophthalmology 030104 developmental biology chemistry Age-Related Macular Degeneration lcsh:RE1-994 Toxicity 030221 ophthalmology & optometry Pyrene Trypan blue Original Article business |
| Zdroj: | Journal of Ophthalmic & Vision Research, Vol 13, Iss 4, Pp 419-425 (2018) Journal of Ophthalmic & Vision Research Journal of ophthalmic & vision research, vol 13, iss 4 |
| Popis: | PurposeThe aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells.MethodsWe pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured.ResultsOur results demonstrated that when treated with B(e)P, the viability and ΔΨm of ARPE-19 cells declined by 25% and 63%, respectively (P < 0.05). However, pretreating with 17β-estradiol increased the viability of ARPE-19 cells by 21% (20 nM) and 10% (40 nM) (P < 0.05). Furthermore, the significantly reduced ΔΨm in βE+B(e)P treated cells ARPE-19 cells was restored by pre-treatment with 17β-estradiol- ΔΨm was increased by 177% (20 nM) and 158% (40 nM) (P < 0.05). We further observed a significant up-regulation in the activity of Caspases-3/7, -9, and -12 in B(e)P-treated ARPE-19 cells. However, 17β-estradiol treatment significantly reduced the activity of all apoptotic markers (P < 0.05).ConclusionIn conclusion, our results demonstrate that 17β-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential. |
| Databáze: | OpenAIRE |
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