The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress
Autor: | P. Yaffe, Asher Ornoy, Y Shoenfeld, Zivanit Ergaz, Gil Goldzweig, Michael B. Blank, N. Patlas, A. Cohen, Dror Mevorach |
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Rok vydání: | 2010 |
Předmět: |
Male
medicine.medical_specialty Antioxidant food.ingredient medicine.medical_treatment Low molecular weight antioxidants medicine.disease_cause Antibodies Antioxidants food Rheumatology Antiphospholipid syndrome Pregnancy Internal medicine Yolk medicine Animals Humans Lupus Erythematosus Systemic business.industry Superoxide Dismutase Embryo medicine.disease Antiphospholipid Syndrome Catalase Embryo Mammalian Rats Specific antibody Oxidative Stress Endocrinology embryonic structures Female Post implantation business Oxidative stress |
Zdroj: | Lupus. 19(14) |
ISSN: | 1477-0962 |
Popis: | We evaluated the embryolethality and embryotoxicity of sera from patients suffering from autoimmune diseases during remission on post-implantation rat embryos cultured on these sera and determined the association between the patients’ clinical history, high blood levels of specific antibodies, medications, and oxidative stress parameters. One hundred and eighty, 10.5-day-old rat embryos were cultured in their yolk sacs in 33 sera of systemic lupus erythematosus (SLE)/antiphospholipid syndrome (APS) patients, and compared with 84 embryos cultured in rat sera and 88 embryos cultured in control human sera. The sera proved to be lethal and embryotoxic but not teratogenic resulting in smaller yolk sacs and embryos, lower protein level and lower developmental score. Significantly less embryos cultured in ‘toxic’ SLE/APS sera had peak 2 of low molecular weight antioxidants (LMWA) wave, implying a delayed maturation of the antioxidant defense. Lower peak 1 of LMWA correlated with a history of recurrent abortions. Embryonic levels of superoxide dismutase (SOD) and catalase (CAT) did not correlate with sera toxicity, patients’ clinical history or specific antibodies. We conclude that SLE/APS patients’ clinical remission did not prevent death or developmental delay accompanied by later appearance of peak 2 of LMWA in post-implantation rat embryo cultures. The normal levels of the antioxidant enzymes evaluated may indicate that sera toxicity is not related to oxidative stress. Lupus (2010) 19, 1623—1631. |
Databáze: | OpenAIRE |
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