Triptolide reduces proliferation and enhances apoptosis of human non-small cell lung cancer cells through PTEN by targeting miR-21
| Autor: | Wufang Fan, Youchao Jia, Ran Huo, Aimin Zang, Mindan Duan, Lei Zhang, Xiaofang Li, Jin Jiao, Jia Feng, Xiaowei Zhu, Jinchao Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Lung Neoplasms Cell Survival Cell Antineoplastic Agents Apoptosis Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Carcinoma Non-Small-Cell Lung Cell Line Tumor Genetics medicine Humans Tensin PTEN Molecular Biology Cell Proliferation Oncogene Cell growth PTEN Phosphohydrolase Phenanthrenes Triptolide Cell cycle Cell biology Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology chemistry 030220 oncology & carcinogenesis Cancer research biology.protein Epoxy Compounds Molecular Medicine RNA Interference Diterpenes Drug Screening Assays Antitumor |
| Zdroj: | Molecular Medicine Reports. 13:2763-2768 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2016.4844 |
| Popis: | Triptolide is used in traditional Chinese medicine. It has the advantages of a unique mechanism of action, a wide antitumor spectrum, multiple targets, multi-channel effects and low toxicity. The current study was conducted to evaluate whether the potential anticancer effects of triptolide reduces proliferation and enhances apoptosis of human non‑small cell lung cancer (NSCLC) cells, and to assess the underlying anticancer mechanisms. In PC‑9 cells, treatment with triptolide reduced cell proliferation and increased cell apoptosis and caspase‑3 and 9 activity. Triptolide treatment reduced miR‑21 expression and enhanced phosphatase and tensin homolog (PTEN) protein expression levels in the PC‑9 cells. Furthermore, the upregulation of miR‑21 expression levels suppressed the effect of triptolide on cell viability and PTEN protein expression levels in PC‑9 cells. To the best of our knowledge, the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21. |
| Databáze: | OpenAIRE |
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