Laminin alpha 5, a major transcript of normal and malignant rat liver epithelial cells, is differentially expressed in developing and adult liver
| Autor: | Ernesto G. Bade, Thomas Seebacher, J.L. Medina |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Gene isoform
Biology Immunoglobulin light chain Polymerase Chain Reaction Cell Line Extracellular matrix Fetus Liver Neoplasms Experimental Laminin Tumor Cells Cultured Animals Hepatectomy Cell Line Transformed chemistry.chemical_classification Nucleic acid sequence Gene Expression Regulation Developmental Epithelial Cells Cell Biology Molecular biology Liver Regeneration Rats Genes ras chemistry Liver Urinary Bladder Neoplasms Cell culture biology.protein Glycoprotein |
| Zdroj: | Experimental cell research. 237(1) |
| ISSN: | 0014-4827 |
| Popis: | The laminin family of extracellular matrix glycoproteins plays a major role in cell migration and differentiation and in tumor cell invasion. As previously shown, the laminin deposited by normal and malignant rat liver epithelial cells in their extracellular matrix (ECM) and into their ECM migration tracks does not contain a typical (EHS-like) alpha 1 heavy chain. By RT-PCR screening we have now identified two alpha chains among a total of five additional laminin chains produced by these cells. Three of the newly identified chains were not previously known for the rat. Their sequences have been deposited in the EMBL nucleotide sequence data bank. The alpha 5 chain now identified is expressed at comparably high levels by both the normal and the malignant liver epithelial cells. The chain is also expressed in fetal liver together with the alpha 2 and beta 2 chains, but it is only vestigially expressed in the mature organ as shown by RT-PCR. These results suggest for alpha 5 a role in development and production of the chain by only a small subset of cells in adult liver. At the level of detection used, no changes were observed in regenerating liver after partial hepatectomy. In addition to the alpha 5 chain, the cultured cells express the beta 1 and beta 2 light chains, indicating the expression of more than one laminin isoform by the same cell line. The expression of the alpha 5 chain and of the other new non-EHS isoform chains was also analyzed in various tissues. The malignant liver epithelial cells, but not their nontumorigenic parental cells, also express, in addition to the alpha 5 chain the alpha 2 chain, which is expressed at high level by the NBT II bladder carcinoma cell line, suggesting a relationship with malignancy. |
| Databáze: | OpenAIRE |
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