Coal-Degrading Bacteria Display Characteristics Typical of Plant Growth Promoting Rhizobacteria
| Autor: | A. Keith Cowan, Wiya L. Masudi, Lerato M. Sekhohola-Dlamini, Yinka Titilawo, Jacob Taiwo Olawale |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbacterium Bioengineering 010501 environmental sciences Rhizobacteria phylogeny lcsh:Chemical technology 01 natural sciences Serratia complex mixtures biodegradation lcsh:Chemistry 03 medical and health sciences diesel-contaminated soil Chemical Engineering (miscellaneous) lcsh:TP1-1185 Food science 0105 earth and related environmental sciences Citrobacter Rhizosphere biology Chemistry Process Chemistry and Technology plant growth-promoting bacteria food and beverages Exiguobacterium biology.organism_classification respiratory tract diseases Proteus 030104 developmental biology lcsh:QD1-999 discard dumps coal discard Bacteria |
| Zdroj: | Processes, Vol 8, Iss 1111, p 1111 (2020) Processes Volume 8 Issue 9 |
| ISSN: | 2227-9717 |
| Popis: | Coal mining produces large quantities of discard that is stockpiled in large dumps. This stockpiled material, termed coal discard, poses an environmental threat emphasising the need for appropriate bioremediation. Here, metagenomic analysis of the 16S rRNA from ten coal-degrading strains previously isolated from coal slurry from discard dumps and from the rhizosphere of diesel-contaminated sites was used to establish genetic relatedness to known plant growth-promoting (PGP) bacteria in the NCBI database. Measurement of indole and ammonium production and solubilisation of P and K were used to screen bacteria for PGP characteristics. BLAST analysis revealed &ge 99% homology of six isolates with reference PGP strains of Bacillus, Escherichia, Citrobacter, Serratia, Exiguobacterium and Microbacterium, while two strains showed 94% and 91% homology with Proteus. The most competent PGP strains were Proteus strain ECCN 20b, Proteus strain ECCN 23b and Serratia strain ECCN 24b isolated from diesel-contaminated soil. In response to L-trp supplementation, the concentration of indolic compounds (measured as indole-3-acetic acid) increased. Production of ammonium and solubilisation of insoluble P by these strains was also apparent. Only Serratia strain ECCN 24b was capable of solubilising insoluble K. Production of indoles increased following exposure to increasing aliquots of coal discard, suggesting no negative effect of this material on indole production by these coal-degrading bacterial isolates and that these bacteria may indeed possess PGP characteristics. |
| Databáze: | OpenAIRE |
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