Porous alginate/poly(ε-caprolactone) scaffolds: preparation, characterization and in vitro biological activity
| Autor: | Mara Tommasini, Maria Teresa Conconi, Pier Paolo Parnigotto, Chiara Giraudo, Daniele Dalzoppo, Piergiorgio Paganin, Rosa Di Liddo, Giampietro Feltrin, Claudio Grandi, Silvano Lora |
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| Rok vydání: | 2010 |
| Předmět: |
Biocompatibility
Alginates Polyesters Bone and Bones Extracellular matrix chemistry.chemical_compound Mice Calcification Physiologic bone regeneration Glucuronic Acid Cell Line Tumor scaffold porosity Genetics Animals calcium alginate threads Bone regeneration Porosity Tissue Scaffolds poly(epsilon-caprolactone) Hexuronic Acids Biomaterial General Medicine X-Ray Microtomography Molecular biology bone regeneration scaffold porosity poly(epsilon-caprolactone) calcium alginate threads scaffold pore size chemistry Chemical engineering Cell culture scaffold pore size Ethylene glycol Caprolactone |
| Zdroj: | International journal of molecular medicine. 27(3) |
| ISSN: | 1791-244X |
| Popis: | In bone tissue engineering, scaffolds with controlled porosity are required to allow cell ingrowth, nutrient diffusion and sufficient formation of vascular networks. The physical properties of synthetic scaffolds are known to be dependent on the biomaterial type and its processing technique. In this study, we demonstrate that the separation phase technique is a useful method to process poly(e-caprolactone) (PCL) into a desired shape and size. Moreover, using poly(ethylene glycol), sucrose, fructose and Ca2+ alginate as porogen agents, we obtained PCL scaffolds with three-dimensional porous structures characterized by different pore size and geometry. Scanning electron microscopy and porosity analysis indicated that PCL scaffolds prepared with Ca2+ alginate threads resemble the porosity and the homogeneous pore size distribution of native bone. In parallel, MicroCT analysis confirmed the presence of interconnected void spaces suitable to guarantee a biological environment for cellular growth, as demonstrated by a biocompatibility test with MC3T3-E1 murine preosteoblastic cells. In particular, scaffolds prepared with Ca2+ alginate threads increased adhesion and proliferation of MC3T3-E1 cells under basal culture conditions, and upon stimulation with a specific differentiation culture medium they enhanced the early and later differentiated cell functions, including alkaline phosphatase activity and mineralized extracellular matrix production. These results suggest that PCL scaffolds, obtained by separation phase technique and prepared with alginate threads, could be considered as candidates for bone tissue engineering applications, possessing the required physical and biological properties. |
| Databáze: | OpenAIRE |
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