Simultaneous determination of fourteen components of Gumiganghwal-tang tablet in human plasma by UPLC-ESI-MS/MS and its application to pharmacokinetic study
| Autor: | Hea-Young Cho, Ji-Hun Jang, Yong-Bok Lee, Seung-Hyun Jeong, Seung-Jung Yang, Guk-Yeo Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Formic acid
Electrospray ionization Pharmaceutical Science 02 engineering and technology Pharmacy RM1-950 01 natural sciences High-performance liquid chromatography Analytical Chemistry Clinical study chemistry.chemical_compound Pharmacokinetics Drug Discovery Electrochemistry Simultaneous analysis Spectroscopy Fourteen bioactive ingredients Active ingredient Chromatography Elution 010401 analytical chemistry Selected reaction monitoring 021001 nanoscience & nanotechnology 0104 chemical sciences Triple quadrupole mass spectrometer Gumiganghwal-tang chemistry Original Article Therapeutics. Pharmacology UPLC-ESI-MS/MS 0210 nano-technology |
| Zdroj: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Vol 11, Iss 4, Pp 444-457 (2021) |
| ISSN: | 2214-0883 2095-1779 |
| Popis: | Gumiganghwal-tang is a traditional herbal medicine widely used for its anti-inflammatory, analgesic, and antipyretic effects. However, the safety and efficacy of its active ingredients based on an in vivo pharmacokinetic (PK) study have yet been investigated. We have established a sensitive and accurate UPLC-ESI-MS/MS method and conducted a PK study on 14 constituents of Gumiganghwal-tang through human plasma analysis. Analytical conditions were optimized according to the physicochemical properties of the 14 compounds to facilitate efficient separation and eliminate overlap or interference between peaks. KINETEX-C18 and Inertsil-C8 columns were used as UPLC stationary phases, and acetonitrile and aqueous formic acid were used as mobile phases. All the analytes were quantified with a triple quadrupole mass spectrometer using electrospray ionization in multiple reaction monitoring mode. The chromatograms of 14 bioactive compounds showed excellent elution and sensitivity, and each peak was selectively separated and quantified without interference with each other or impurities. The established analytical method was based on international guidelines and was successfully used to perform PK studies of 14 herbal ingredients in humans after oral administration with Gumiganghwal-tang tablets. The oral absorption of most active components of Gumiganghwal-tang was relatively rapid and remained considerably long in the body to be quantified in plasma up to 48 h after administration. Graphical abstract Image 1 Highlights • Establishment and validation of simultaneous analysis of Gumiganghwal-tang components. • Quantification of 14 major components of Gumiganghwal-tang from human plasma samples. • Exploring the pharmacokinetic characteristics of Gumiganghwal-tang tablet in humans. |
| Databáze: | OpenAIRE |
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