Correction: Profiles of a broad spectrum of epigenetic DNA modifications in normal and malignant human cell lines: Proliferation rate is not the major factor responsible for the 5-hydroxymethyl-2'-deoxycytidine level in cultured cancerous cell lines
| Autor: | Ryszard Olinski, Magdalena Skonieczna, Karol Bialkowski, Dorota Hudy, Marek Foksinski, Anna Labejszo, Karolina Gajda, Marta Starczak, Ewelina Zarakowska, Daniel Gackowski, Joanna Rzeszowska-Wolny, Anna Szpila, Jarosław Czyż |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Lines lcsh:Medicine Research and Analysis Methods Biochemistry Hematologic Cancers and Related Disorders 03 medical and health sciences chemistry.chemical_compound hemic and lymphatic diseases Oxidation Epigenetic Profile Genetics Medicine and Health Sciences Epigenetics lcsh:Science Colorectal Cancer Multidisciplinary lcsh:R Chemical Reactions Biology and Life Sciences Cancers and Neoplasms Burkitt's Lymphoma DNA Hematology Cell Cultures Molecular biology Nucleic acids Chemistry 030104 developmental biology DNA demethylation Real-time polymerase chain reaction chemistry Oncology Cell culture Cancer cell Physical Sciences Deoxycytidine Lymphomas lcsh:Q Biological Cultures Gene expression Cultured Fibroblasts DNA modification Raji Cells Research Article |
| Zdroj: | PLoS ONE, Vol 13, Iss 4, p e0195819 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Active demethylation of 5-methylcytosine moiety in DNA occurs by its sequential oxidation to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxycytosine, catalysed by enzymes of the Ten-Eleven Translocation family proteins (TETs 1, 2 and 3). Here we analyzed for the first time all the intermediate products of DNA demethylation pathway in the form of deoxynucleosides (5-methyl-2'-deoxycytidine, 5-(hydroxymethyl)-2'-deoxycytidine, 5-formyl-2'-deoxycytidine and 5-carboxy-2'-deoxycytidine as well as 5-(hydroxymethyl)-2'-deoxyuridine) using automated isotope-dilution online two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry. DNA was isolated from human malignant cell lines of colon adenocarcinoma (HCT 116), melanoma (Me45), myelogenous leukemia bone marrow blasts (K562), EBV-positive Burkitt's lymphoma lymphoblasts (Raji), EBV-negative Burkitt's lymphoma lymphoblasts (male-CA46 and female-ST486), as well as normal neonatal dermal fibroblasts (NHDF-Neo). The expression levels of TET1, TET2, TET3, SMUG1, and TDG genes were also assayed by RT-qPCR. Our results show a global erasure of 5-hydroxymethyl-2'-deoxycytidine and 5-carboxy-2'-deoxycytidine in DNA of cultured cells compared with DNA from primary malignant tissue. Moreover, malignant cells in culture have a quite different DNA epigenetic profile than cultured normal cells, and different types of malignant cells display different and characteristic profiles of DNA epigenetic marks. Similar analyses of a broader spectrum of epigenetic modifications, not restricted to 5-methyl-2'-deoxycytidine, could lead to better understanding of the mechanism(s) responsible for emergence of different types of cancer cells. |
| Databáze: | OpenAIRE |
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