Sensitive determination of trimetazidine in spiked human plasma by HPLC with fluorescence detection after pre-column derivatization with 9-fluorenylmethyl chloroformate
| Autor: | Alaa Khedr, Ibrahim A. Darwish, Mahmoud Sheha |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Vasodilator Agents
Clinical Biochemistry Trimetazidine Chloroformate Sensitivity and Specificity Biochemistry High-performance liquid chromatography Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Humans Derivatization Acetonitrile Chromatography High Pressure Liquid Detection limit Fluorenes Chromatography Reproducibility of Results Cell Biology General Medicine Spectrometry Fluorescence chemistry Calibration Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Sodium acetate |
| Zdroj: | Journal of Chromatography B. 856:337-342 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2007.06.036 |
| Popis: | A high-performance liquid chromatographic method for the determination of trimetazidine dihydrochloride (TMZ) in spiked human plasma is described. The method is based on the pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) using the fluorimetric detection technique. Fluoxetine HCl (FLX) was used as internal standard. Both, TMZ and FLX were completely derivatized after heating at 50 degrees C for 20 min in borate buffer pH 8.0. Samples were analyzed by high performance liquid chromatography (HPLC) using Zorbax-TMS column (250 mm x 4.6 mm, i.d., 5 microm) and mobile phase consist of acetonitrile, methanol and 20 mM sodium acetate pH 4.7 (44:6:50; v/v/v). Fluorescence detector (FLD) was adjusted at excitation and emission wavelengths; 265 and 311 nm, respectively. The linearity of the method was in the range of 4.5-200 ng/ml. Limits of detection (LOD) and quantification (LOQ) were 1.5 and 4.5 ng/ml, respectively. Trimetazidine recovery was 96.5+/-1.3% (n=6; RSD=2.1%). |
| Databáze: | OpenAIRE |
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