Size-exclusion high-performance liquid chromatography of integral membrane proteins; effect of detergents on immunological activity
| Autor: | B. Kazemier, G. W. Welling, S. Welling-Wester |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Chromatography
biology Chemistry Organic Chemistry Clinical Biochemistry Size-exclusion chromatography biology.organism_classification Biochemistry High-performance liquid chromatography Sendai virus Analytical Chemistry Column chromatography Protein structure Membrane protein Protein purification Integral membrane protein |
| Zdroj: | Chromatographia, 24, 790-794 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02688586 |
| Popis: | A mixture of integral membrane proteins from Sendai virus was used as a model to study the effect of various detergents and organic solvent on the purification by size-exclusion high-performance liquid chromatography (HPLC) on two tandemly linked Superose-6 columns. The best separations were obtained with either 0.1% sodium dodecyl sulphate, 0.05% sarkosyl or 0.1% lauryldimethylamineoxide in the eluent. In addition, the effect of the eluent on the immunological activity as a measure of intact structure was studied. A considerable part of the protein structure remained intact after HPLC and was able to react with antibodies directed against the intact virus proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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