Detection of bile salt-dependent lipase, a 110 kDa pancreatic protein, in urines of healthy subjects
| Autor: | Marie-Odile Sadoulet, A. Nganga, Françoise Silvy, B. Comte, Dominique Lombardo, Eric Mas, Cécile Franceschi, D. Lafitte, Jean-Paul Bernard, L. Daniel, Benkoel L |
|---|---|
| Přispěvatelé: | Cytosquelette et Intégration des Signaux du Micro-Environnement Tumoral - UMR 6032 (CISMET), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Université de la Méditerranée - Aix-Marseille 2, Vidalin, Michèle, Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Předmět: |
Adult
Male Proteomics medicine.medical_specialty Renal glomerulus Dietary lipid Kidney Glomerulus Molecular Sequence Data Biology Mass Spectrometry 03 medical and health sciences 0302 clinical medicine In vivo Internal medicine medicine Humans Immunoprecipitation Amino Acid Sequence pancreas Kidney Tubules Collecting 030304 developmental biology chemistry.chemical_classification 0303 health sciences Biological Transport Middle Aged Sterol Esterase Bile salt-dependent lipase Immunohistochemistry In vitro urine 3. Good health medicine.anatomical_structure Endocrinology Transcytosis chemistry Nephrology 030220 oncology & carcinogenesis biology.protein Electrophoresis Polyacrylamide Gel Pancreas Glycoprotein Filtration Protein Binding bile salt-dependent lipase |
| Zdroj: | Kidney International Kidney International, Nature Publishing Group, 2006, 69(6), pp.1048-55 Kidney International, 2006, 69(6), pp.1048-55 |
| ISSN: | 0085-2538 1523-1755 |
| DOI: | 10.1038/sj.ki.5000133 |
| Popis: | Bile salt-dependent lipase (BSDL), a 110 kDa glycoprotein secreted by the pancreatic acinar cells, participates in the duodenal hydrolysis of dietary lipid esters. Recent in vitro and in vivo studies demonstrated that the BSDL reaches the blood via a transcytosis motion through enterocytes, suggesting that this enzyme may play a role in vascular biology. Once in the blood, BSDL should be eliminated. We address the hypothesis that BSDL may be filtered by the glomerulus and eliminated in urines. Immunological methods and proteomic were used to detect and to characterize BSDL in urine. The immunoreactive form of BSDL was detected in urines of 36 male subjects devoid of renal failure. Proteomic demonstrated that the immunoreactive protein is BSDL. Experiments using a monoclonal antibody to the oncofetal glycoform of pancreatic BSDL suggested that the protein is not expressed by renal cells but originates from the pancreas via circulation. We demonstrate that under normal physiological conditions, BSDL, a high-molecular weight blood glycoprotein, can be filtered by the renal glomerulus to be eliminated in urines. |
| Databáze: | OpenAIRE |
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