Effects of different forms of chitosan on intercellular junctions of mouse fibroblasts in vitro
| Autor: | Suna Özbaş-Turan, Ayhan Bilir, Seçnur Denir, Bahar Uslu, Burcu Biltekin, Jülide Akbuğa, Serap Arbak |
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| Rok vydání: | 2015 |
| Předmět: |
Histology
Cell Survival macromolecular substances 02 engineering and technology 010402 general chemistry Polysaccharide 01 natural sciences Cell junction 3T3 cells Chitosan Mice chemistry.chemical_compound Microscopy Electron Transmission medicine Animals MTT assay chemistry.chemical_classification ICAM-1 Chemistry technology industry and agriculture 3T3 Cells General Medicine Fibroblasts equipment and supplies 021001 nanoscience & nanotechnology Immunohistochemistry Molecular biology In vitro 0104 chemical sciences carbohydrates (lipids) Medical Laboratory Technology Intercellular Junctions medicine.anatomical_structure Cell culture 0210 nano-technology |
| Zdroj: | Biotechnic & Histochemistry. 91:20-29 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.3109/10520295.2015.1064998 |
| Popis: | Chitosan is a linear polysaccharide that has many biomedical applications. We compared the effects of chitosan, in both solution and membranous form, on intercellular adhesion of Swiss 3T3 mouse fibroblasts. Cells were grown as spheroidal cell cultures. Some control cell spheroids were cultured without chitosan and two experimental groups were cultured with chitosan. Chitosan in solution was used for one experimental group and chitosan in membranous form was used for the other. For each group, intercellular adhesion was investigated on days 5 and 10 of culture. Transmission electron microscopy revealed well-defined cellular projections that were more prominent in cells exposed to either membranous or solution forms of chitosan than to the chitosan-free control. Immunocytochemical staining of ICAM-1 and e-cadherin was used to determine the development of intercellular junctions. Compared to the weakly stained control, strong reactions were observed in both chitosan exposed groups at both 5 and 10 days. Cells were treated with 5-bromo-2-deoxyuridine (BrdU) and incubated with anti-BrdU primary antibody to assess proliferation. Both the solution and membranous forms of chitosan increased proliferation at both 5 and 10 days. Cellular viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The MTT assay indicated high cell viability; maximum viability was obtained with the solution form of chitosan at day 5. Chitosan exposure increased the number of intercellular junctions and showed a significant proliferative effect on 3T3 mouse fibroblasts. |
| Databáze: | OpenAIRE |
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