Comparison of SARS-CoV-2 antigen electrochemiluminescence immunoassay to RT-PCR assay for laboratory diagnosis of COVID-19 in Peshawar
| Autor: | Mohsin Shafi, Maria Khan, N. H. Shah, Bilal Iqbal, Mirza Muhammad Dawood, Valeed Jehanzeb |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Clinical Biochemistry Medicine (miscellaneous) Sensitivity and Specificity Gastroenterology COVID-19 Testing Antigen Internal medicine medicine Humans Electrochemiluminescence Immunoassay medicine.diagnostic_test Clinical Laboratory Techniques Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 business.industry Health Policy Biochemistry (medical) Public Health Environmental and Occupational Health COVID-19 Gold standard (test) Cross-Sectional Studies Real-time polymerase chain reaction business Viral load |
| Zdroj: | Diagnosis. 9:364-368 |
| ISSN: | 2194-802X 2021-0078 |
| DOI: | 10.1515/dx-2021-0078 |
| Popis: | Objectives Antigen based rapid diagnostic tests possesses a potential to be utilized along with Gold standard methods to detect Covid-19 infection to cope with the demand of testing. The aim of this study was to determine diagnostic accuracy of electrochemiluminescence based automated antigen detection immunoassay comparing with molecular based test RT-PCR (Covid-19). Methods It was a cross-sectional study conducted in RMI Peshawar, from 1st April 2021 till 30th April 2021. The study comprised 170 individuals who were suspected of having Covid-19. Nasopharyngeal samples taken from suspected individuals were analyzed by RT-PCR and automated antigen test (Elecsys SARS-CoV-2 Antigen) simultaneously. The correlation of SARS-CoV-2 antigen with PCR positive and negative cases was analyzed for specificity, sensitivity respectively. Results The ECLIA based Elecsys antigen test (Roche) revealed overall sensitivity 72%, specificity 95% and accuracy of 94.9%. Sensitivity of antigen test progressively declined from 94.3% in Ct Conclusions Based on the findings of our study we conclude that automated antigen testing (Elecsys SARS-CoV-2 Antigen) cannot replace molecular based testing like RT PCR. Elecsys SARS-CoV-2 Ag test should be used complementary to RT-PCR in testing algorithms. Frequent testing strategy should be adopted while using automated antigen testing to overcome its limitation in individuals with low viral loads. |
| Databáze: | OpenAIRE |
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