Effects of fusaric acid treatment on the protocorm-like bodies of Dendrobium sonia-28
| Autor: | Sreeramanan Subramaniam, Azhar Mohamad, Alireza Joniyas, Latiffah Zakaria, Raheleh Dehgahi |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Fusarium proliferatum Plant Science 01 natural sciences Somaclonal variation Plantlet Dendrobium 03 medical and health sciences chemistry.chemical_compound Microscopy Electron Transmission Cell Wall Botany Plant Diseases biology Cell Biology General Medicine Fusaric Acid 030108 mycology & parasitology biology.organism_classification RAPD Random Amplified Polymorphic DNA Technique Horticulture chemistry Shoot Microscopy Electron Scanning Fusaric acid Plant Shoots 010606 plant biology & botany |
| Zdroj: | Protoplasma. 253(5) |
| ISSN: | 1615-6102 |
| Popis: | Dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. Unfortunately, it is being decimated by fungal diseases, especially those caused by Fusarium proliferatum. In this study, selection of F. proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of differing concentrations of fusaric acid (FA). PLBs were cultured on Murashige and Skoog (MS) medium supplemented with 0.05 to 0.2 millimolar (mM) concentrations of FA. Higher concentrations of FA increased mortality of PLBs and reduced their growth. The survival rate for 0.05 mM FA was 20 % but only 1 % at the highest dose of 0.2 mM. Additionally, two different size ranges of PLBs were investigated, and growth increased more at lower FA concentrations for larger PLBs, whilst the growth rate of smaller PLBs was inhibited at an FA concentration of 0.2 mM. Histological examination using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses disclosed severe cell wall and organelle damage, as well as stomatal closure in PLBs treated with the high FA concentrations. Reductions in plantlet growth were much greater at the highest concentrations of FA. Some randomly amplified polymorphic DNA (RAPD) markers clearly discriminated between selected and non-selected variants of Dendrobium sonia-28, showing different banding patterns for each FA concentration and specific bands for selected and control plants. |
| Databáze: | OpenAIRE |
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