Molecular and cytogenetic studies of a patient with Philadelphia-negative, BCR-positive chronic myeloid leukemia and t(12;12)(q13;p12)
| Autor: | Alfonso Zaccaria, Nicoletta Testoni, Angela Tassinari, Bommina Celso, Beverly S. Emanuel, Marcia Budarf, Giuseppe Saglio, Angelo Guerrasio, Cosimo Barletta, Cesare Peschle, Sante Tura |
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| Rok vydání: | 1990 |
| Předmět: |
Adult
Electrophoresis Agar Gel Cancer Research ABL breakpoint cluster region Myeloid leukemia Nucleic Acid Hybridization Chromosomal translocation Karyotype In situ hybridization DNA Neoplasm Biology Leukemia Myeloid Chronic Atypical BCR-ABL Negative Translocation Genetic Blotting Southern Regulatory sequence hemic and lymphatic diseases Karyotyping Leukemia Myelogenous Chronic BCR-ABL Positive Genetics Cancer research Humans Female K562 cells |
| Zdroj: | Genes, chromosomescancer. 1(4) |
| ISSN: | 1045-2257 |
| Popis: | A patient with Philadelphia (Ph1)-negative, breakpoint cluster region (bcr)-positive chronic myeloid leukemia (CML) is reported. Pulsed-field gel electrophoretic analysis demonstrated the comigration of both ABL and BCR sequences on the same BssHII and SacII fragment. Moreover, in situ hybridization studies demonstrated that ABL sequences had been moved from band 9q34 to 22q11 and that the additional t(12;12)(q13;p12) was not involved in the ABUBCR related translocation. Nevertheless, a possible role of oncogenes or regulatory sequences activated or inhibited by the additional translocation cannot be excluded. |
| Databáze: | OpenAIRE |
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