Diagnostic Microbiology and Infectious Disease
| Autor: | Elizabete de Jesus Inês, Neci Matos Soares, Joelma Nascimento de Souza, Mônica L. S. Silva, Márcia Cristina Aquino Teixeira |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Glycosylation Antibodies Helminth Enzyme-Linked Immunosorbent Assay Sodium metaperiodate Cross Reactions Epitope Strongyloides stercoralis Epitopes Feces Antigen Animals Humans Serologic Tests Parasite Infections In patient Pathogen Glycoproteins chemistry.chemical_classification Serodiagnosis biology Periodic Acid General Medicine Immunoglobulin E biology.organism_classification Virology Infectious Diseases chemistry Antigens Helminth Immunoglobulin G Larva Strongyloidiasis Schistosoma mansoni Glycoprotein |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2013.01.016 |
| Popis: | Texto completo. Acesso restrito. p. 31-35 Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-06-12T18:06:42Z No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5) Approved for entry into archive by Alda Lima da Silva(sivalda@ufba.br) on 2013-06-18T20:41:48Z (GMT) No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5) Made available in DSpace on 2013-06-18T20:41:48Z (GMT). No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5) Previous issue date: 2013 Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzymelinked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis–infected patients. Salvador |
| Databáze: | OpenAIRE |
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