Dynamic and Functional Profiling of Xylan-Degrading Enzymes in Aspergillus Secretomes Using Activity-Based Probes
| Autor: | Eric Abbate, Jos Reijngoud, Lluís Raich, Herman S. Overkleeft, Nicholas McGregor, Olga V. Moroz, Arthur F. J. Ram, Gilles P. van Wezel, Sybrin P. Schröder, Liang Wu, Bogdan I. Florea, Mary Ann Stringer, Jeroen D. C. Codée, Casper de Boer, Jean-Guy Berrin, Rhianna J. Rowland, Marc Dominique Morant, Carme Rovira, David Osborn, Kristian B. R. M. Krogh, Elena Blagova, Mark Arentshorst, Keith S. Wilson, Gijsbert A. van der Marel, Gideon J. Davies |
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| Přispěvatelé: | Wu, Liang, Davies, Gideon J., Overkleeft, Herman S., Universiteit Leiden, York Structural Biology Laboratory, Department of Chemistry, University of York [York, UK], Novozymes, Inc., Partenaires INRAE, Novozymes A/S, Institució Catalana de Recerca i Estudis Avançats (ICREA), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Leiden University, Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
[SDV]Life Sciences [q-bio] enzymes 010402 general chemistry Polysaccharide 01 natural sciences glycoside hydrolases biomass breakdown β-glucosidase inhibitor [SDV.IDA]Life Sciences [q-bio]/Food engineering [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering QD1-999 chemistry.chemical_classification Aspergillus biology 010405 organic chemistry Aspergillus niger General Chemistry biology.organism_classification Xylan 0104 chemical sciences Protein profiling Chemistry Cyclophellitol Enzyme chemistry Biochemistry Functional profiling |
| Zdroj: | ACS Central Science, 5(6), 1067-1078 ACS Central Science, . (2019) ACS Central Science ACS Central Science, 2019, ⟨10.1021/acscentsci.9b00221⟩ ACS Central Science, ACS Publications, 2019, ⟨10.1021/acscentsci.9b00221⟩ ACS Central Science, Vol 5, Iss 6, Pp 1067-1078 (2019) |
| ISSN: | 2374-7951 2374-7943 |
| Popis: | International audience; Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired by the β-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme–substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown. |
| Databáze: | OpenAIRE |
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