Viral isolation analysis of SARS-CoV-2 from clinical specimens of COVID-19 patients
| Autor: | Satoshi Nomura, Kojiro Hirota, Hideki Tani, Norikazu Hirano, Hitoshi Sasajima, Shin'ichi Takeda, Yumiko Saga, Noriko Inasaki, Yukio Horie, Masae Itamochi, Emiko Igarashi, Takahisa Shimada, Shunsuke Yazawa, Tomomi Ichikawa, Hiroyasu Kaya, Hiroyuki Itoh, Yoshihiro Yamamoto, Sumiyo Hasegawa, Kosuke Tamura, Chikako Kawashiri, Kazunori Oishi, Seiji Yamashiro |
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| Rok vydání: | 2021 |
| Předmět: |
Threshold cycle (Ct) value
Microbiology (medical) Coronavirus disease 2019 (COVID-19) viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Clinical specimens Biology urologic and male genital diseases medicine.disease_cause Real-Time Polymerase Chain Reaction TMPRSS2 Article Virus law.invention Cell Line Virus isolation law VeroE6/TMPRSS2 medicine Humans Pharmacology (medical) Polymerase chain reaction Cytopathic effect Diagnostic Tests Routine SARS-CoV-2 COVID-19 Virology Reverse transcriptase Infectious Diseases Herpes simplex virus |
| Zdroj: | Journal of Infection and Chemotherapy |
| ISSN: | 1437-7780 |
| Popis: | Genetic testing using reverse transcriptase real-time polymerase chain reaction (rRT-PCR) is the mainstay of diagnosis of COVID-19. However, it has not been fully investigated whether infectious viruses are contained in SARS-CoV-2 genome-positive specimens examined using the rRT-PCR test. In this study, we examined the correlation between the threshold Cycle (Ct) value obtained from the rRT-PCR test and virus isolation in cultured cells, using 533 consecutive clinical specimens of COVID-19 patients. The virus was isolated from specimens with a Ct value of less than 30 cycles, and the lower the Ct value, the more efficient the isolation rate. A cytopathic effect due to herpes simplex virus type 1 contamination was observed in one sample with a Ct value of 35 cycles. In a comparison of VeroE6/TMPRSS2 cells and VeroE6 cells used for virus isolation, VeroE6/TMPRSS2 cells isolated the virus 1.7 times more efficiently than VeroE6 cells. There was no significant difference between the two cells in the mean Ct value of the detectable sample. In conclusion, Lower Ct values in the PCR test were associated with higher virus isolation rates, and VeroE6/TMPRSS2 cells were able to isolate viruses more efficiently than VeroE6 cells. |
| Databáze: | OpenAIRE |
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