A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal
| Autor: | Cassandra Kelly-Cirino, Alexandra Weirich, Bhabana Shrestha, Bhagwan Maharjan, Andrew Stewart |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine medicine.medical_specialty Standard of care Tuberculosis 030106 microbiology Reference laboratory Smear microscopy Mycobacterium tuberculosis 03 medical and health sciences Nepal Refrigeration Long-term sputum transport medicine Humans Cold chain biology business.industry lcsh:Public aspects of medicine Sputum Culture contamination lcsh:RA1-1270 biology.organism_classification medicine.disease Surgery Original Article OMNIgene SPUTUM medicine.symptom business |
| Zdroj: | Journal of Epidemiology and Global Health, Vol 6, Iss 4, Pp 257-265 (2016) Journal of Epidemiology and Global Health, Vol 6, Iss 4 (2019) Journal of Epidemiology and Global Health |
| ISSN: | 2210-6006 |
| Popis: | This preliminary study evaluated the transport reagent OMNIgene SPUTUM (OMS) in a real-world, resource-limited setting: a zonal hospital and national tuberculosis (TB) reference laboratory, Nepal. The objectives were to: (1) assess the performance of OMS for transporting sputum from peripheral sites without cold chain stabilization; and (2) compare with Nepal’s standard of care (SOC) for Mycobacterium tuberculosis smear and culture diagnostics. Sixty sputa were manually split into a SOC sample (airline-couriered to the laboratory, conventional processing) and an OMS sample (OMS added at collection, no cold chain transport or processing). Smear microscopy and solid culture were performed. Transport was 0–8 days. Forty-one samples (68%) were smear-positive using both methods. Of the OMS cultures, 37 (62%) were positive, 22 (36%) were negative, and one (2%) was contaminated. Corresponding SOC results were 32 (53%), 21 (35%), and seven (12%). OMS “rescued” six (i.e., missed using SOC) compared with one rescue using SOC. Of smear-positives, six SOC samples produced contaminated cultures whereas only one OMS sample was contaminated. OMS reduced culture contamination from 12% to 2%, and improved TB detection by 9%. The results suggest that OMS could perform well as a no cold chain, long-term transport solution for smear and culture testing. The findings provide a basis for larger feasibility studies. |
| Databáze: | OpenAIRE |
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