Su(H)-Mediated Repression Positions Gene Boundaries along the Dorsal-Ventral Axis of Drosophila Embryos
| Autor: | Angelike Stathopoulos, Lijia Ma, Anil Ozdemir, Kevin P. White |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Biology
Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Gene expression Animals Drosophila Proteins Molecular Biology Transcription factor Psychological repression Body Patterning 030304 developmental biology Regulation of gene expression Genetics 0303 health sciences Gene Expression Regulation Developmental Nuclear Proteins Cell Biology Phosphoproteins Hairless Repressor Proteins DNA binding site Drosophila Chromatin immunoprecipitation 030217 neurology & neurosurgery Drosophila Protein Transcription Factors Developmental Biology |
| Zdroj: | Developmental Cell. 31:100-113 |
| ISSN: | 1534-5807 |
| Popis: | SummaryIn Drosophila embryos, a nuclear gradient of the Dorsal (Dl) transcription factor directs differential gene expression along the dorsoventral (DV) axis, translating it into distinct domains that specify future mesodermal, neural, and ectodermal territories. However, the mechanisms used to differentially position gene expression boundaries along this axis are not fully understood. Here, using a combination of approaches, including mutant phenotype analyses and chromatin immunoprecipitation, we show that the transcription factor Suppressor of Hairless, Su(H), helps define dorsal boundaries for many genes expressed along the DV axis. Synthetic reporter constructs also provide molecular evidence that Su(H) binding sites support repression and act to counterbalance activation through Dl and the ubiquitous activator Zelda. Our study highlights a role for broadly expressed repressors, like Su(H), and organization of transcription factor binding sites within cis-regulatory modules as important elements controlling spatial domains of gene expression to facilitate flexible positioning of boundaries across the entire DV axis. |
| Databáze: | OpenAIRE |
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