A microwell culture system that allows group culture and is compatible with human single media
| Autor: | Masahiro Kaneda, Sumi Shimamura, Hirotsune Kaijima, Tomonori Akai, Atsushi Sugawara, Shoko Ieda, Masanori Kagota, S. Matoba, Yoko Sakaguchi, Satoshi Sugimura |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Zygote Embryonic Development Reproductive technology Fertilization in Vitro Biology Embryo Culture Techniques 03 medical and health sciences 0302 clinical medicine Rate of development Genetics medicine Animals Humans Bovine embryo Blastocyst Genetics (clinical) 030219 obstetrics & reproductive medicine Gene Expression Profiling Embryogenesis Obstetrics and Gynecology Embryo Group culture General Medicine Embryo Mammalian Cell biology Embryo Biology Culture Media 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Cattle Female Developmental Biology |
| Zdroj: | SC40201904180002 NARO成果DBa |
| ISSN: | 1573-7330 |
| DOI: | 10.1007/s10815-018-1252-z |
| Popis: | PURPOSE: A microwell culture system that facilitates group culture, such as well-of-the-well (WOW), improves embryonic development in an individual culture. We examined the effect of WOW on embryonic development in vitro with commercially available human single culture media. METHODS: Using four different commercial human single culture media, in vitro development and imprinted gene expression of bovine embryos cultured in WOW were compared to droplet culture (one zygote per drop). To determine the effects of microwell and group culture on embryonic development, different numbers of embryos were cultured in droplet or WOW. Diffusion simulation of accumulating metabolites was conducted using the finite volume method. RESULTS: WOW had a positive effect on bovine embryonic development, regardless of the type of single culture media. Imprinted gene expression was not different between droplet- and WOW-derived blastocysts. The microwell and group cultures in WOW showed a significant positive effect on the rate of total blastocysts and the rate of development to the expanded and hatching blastocyst stages. The assumed cumulative metabolite concentration of WOW with one embryo was 1.47 times higher than that of droplet culture with one embryo. Furthermore, the concentration of WOW with three embryos was 1.54 times higher than that of WOW with one embryo. CONCLUSIONS: In using human single culture media, a microwell culture system that allows group culture could be a powerful clinical tool for improving the success of assisted reproductive technologies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-018-1252-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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