Endoglin (CD105) and VEGF as potential angiogenic and dissemination markers for colorectal cancer

Autor: Ana Nogués, Jose Luis Marijuan, Eunate Gallardo-Vara, Luisa Mª. Botella, Paloma Mate, Maria Paz Zafra, Alfredo Alonso, Mª. Isabel Prieto
Přispěvatelé: Fundación Mutua Madrileña, Nogués, Ana [0000-0002-7027-3165], Gallardo-Vara, Eunate [0000-0003-4733-0878], Zafra, M. Paz [0000-0001-9303-0097], Marijuan, Jose Luis [0000-0003-0029-0389], Botella, Luisa María [0000-0002-6310-2245], Nogués, Ana, Gallardo-Vara, Eunate, Zafra, M. Paz, Marijuan, Jose Luis, Botella, Luisa María
Rok vydání: 2020
Předmět:
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
Colorectal cancer
VEGF receptors
Metastasis
0302 clinical medicine
Surgical oncology
Medicine
Longitudinal Studies
Prospective Studies
Stage (cooking)
Colectomy
Proctectomy
Neovascularization
Pathologic
biology
Endoglin (CD105)
Endoglin
Middle Aged
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
VEGF
Oncology
030220 oncology & carcinogenesis
Colonic Neoplasms
Immunohistochemistry
Female
Colon
lcsh:Surgery
lcsh:RC254-282
Tumor angiogenesis
Colorectal cancer (CRC)
03 medical and health sciences
Biomarkers
Tumor
otorhinolaryngologic diseases
Humans
Soluble Endoglin
Aged
Neoplasm Staging
Rectal Neoplasms
business.industry
Research
Rectum
Cancer
lcsh:RD1-811
medicine.disease
030104 developmental biology
biology.protein
Cancer research
Surgery
business
Biomarkers
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
World Journal of Surgical Oncology, Vol 18, Iss 1, Pp 1-12 (2020)
World Journal of Surgical Oncology
ISSN: 1477-7819
DOI: 10.1186/s12957-020-01871-2
Popis: 12 p.-5 fig.-2 tab.
Background: Colorectal cancer (CRC) is an important current problem concerning public health due to its high incidence and mortality. Advances in molecular and cellular knowledge and the detection of new disease biomarkers are very important to improve prognosis, prediction, and early diagnosis. In this study, we aimed to analyze the gene and protein expression levels of two angiogenic markers, VEGF and soluble Endoglin, during different tumor stages as well as at different stages of cancer treatment, to predict the diagnosis and evolution of colon and rectal cancer.
Material and methods: This study includes 133 CRC patients (93 with colon cancer and 40 with rectal cancer) on which the gene and protein expression of Endoglin (membrane and soluble form) and VEGF were analyzed by molecular and immunohistochemical techniques on different tumor stage samples and plasma obtained preoperatively as well as 3, 6, and 9months after resection of the tumor.
Results: VEGF and Endoglin gene expressions were higher in tumor tissue than in surrounding non-tumoral tissue for both types of cancer. The VEGF levels in plasma were found to decrease in less aggressive tumors, whereas soluble Endoglin was increased in preoperative samples of patients with metastasis. Membrane Endoglin expression was higher on the vascular endothelium of more aggressive tumors. In contrast, Endoglin expression was mainly in the colon epithelium in less aggressive stage tumors
Conclusion: Endoglin and VEGF are proteins with a major role in the tumor angiogenesis process. This study performed with a wide cohort of human samples shows that both proteins seem to be valuable biomarkers in the diagnosis and prognosis of CRC.
The authors thank Laura Barrios (Statistic Department, CSIC) for statistic and bioinformatics support, Marina Arranz Álvarez (IdiPaz Biobank) for technical samples support, “Fundación de Investigación Médica Mutua Madrileña” for its financial support granted to the research project, and to the Coloproctology Unit of the University Hospital La Paz (Madrid) for their collaboration.
Databáze: OpenAIRE
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