Chrysophanol exhibits inhibitory activities against colorectal cancer by targeting decorin
| Autor: | Yongju Xue, Qiangwu Wang, Xiquan Ke, Jianchao Wang, Xiaodong Chen, Min Deng, Le-Rong Xu, Jun Wei |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cell Survival Colorectal cancer Decorin Clinical Biochemistry Mice Nude Anthraquinones Antineoplastic Agents Apoptosis Biochemistry Mice Structure-Activity Relationship 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation In vivo Tumor Cells Cultured otorhinolaryngologic diseases medicine Animals Humans RNA Small Interfering Cell Proliferation Mice Inbred BALB C Gene knockdown Dose-Response Relationship Drug Molecular Structure Chemistry Neoplasms Experimental Cell Biology General Medicine medicine.disease digestive system diseases In vitro 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research Drug Screening Assays Antitumor Colorectal Neoplasms |
| Zdroj: | Cell Biochemistry and Function. 38:47-57 |
| ISSN: | 1099-0844 0263-6484 |
| DOI: | 10.1002/cbf.3445 |
| Popis: | Colorectal cancer (CRC) is a common human malignancy that accounts for 600,000 deaths annually worldwide. Chrysophanol, a naturally occurring anthraquinone compound, exhibits anti-neoplastic effects in various cancer cells. The aim of this study was to explore the biological effects of chrysophanol on CRC cells, and determine the underlying mechanism. Chrysophanol inhibited proliferation of and promoted apoptosis in CRC cells by activating the intrinsic mitochondrial apoptotic pathway. In addition, chrysophanol also suppressed tumor growth in vivo and increased the percentage of apoptotic cells in tumor xenografts, without general toxicity. Proteomic iTRAQ analysis revealed decorin (DCN) as the major target of chrysophanol. DCN was upregulated in the tumor tissues following chrysophanol treatment, and ectopic DCN expression markedly augmented the pro-apoptotic effects of chrysophanol in CRC cells. In contrast, DCN knockdown significantly abrogated chrysophanol-induced apoptosis in CRC cells. Taken together, chrysophanol exerts anti-neoplastic effects in vitro and in vivo in CRC cells by modulating DCN, there by highlighting its therapeutic potential in CRC. |
| Databáze: | OpenAIRE |
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