Ruminant brain ribonucleases: expression and evolution
| Autor: | Wei Zhao, Adriana Furia, Jaap J. Beintema, Peter A. Jekel, M. P. Sasso, Heleen J. Breukelman, E Confalone, Estherlyn Hodge |
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| Přispěvatelé: | Zhao, W, Confalone, E, Breukelman, Hj, Sasso, Mp, Jekel, Pa, Hodge, E, Furia, Adriana, Beintema, J. J. |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Male
GENES RNase P PSEUDOGENES PROTEINS Pseudogene Genetic Vectors Molecular Sequence Data Biophysics Sequence Homology ruminant Biochemistry SEQUENCE Evolution Molecular Mammary Glands Animal Ribonucleases Structural Biology Molecular evolution evolution Escherichia coli Animals BOVINE BRAIN Amino Acid Sequence RNA Messenger Ribonuclease Molecular Biology Gene giraffe Genetics Messenger RNA Sheep PURIFICATION Base Sequence biology Deer gene duplication Brain Ruminants biology.organism_classification Stop codon chevrotain MOLECULAR EVOLUTION Chevrotain brain ribonuclease biology.protein RAT Cattle Electrophoresis Polyacrylamide Gel Female |
| Zdroj: | Biochimica et biophysica acta-Protein structure and molecular enzymology, 1547(1), 95-103 |
| ISSN: | 0167-4838 |
| Popis: | Molecular evolutionary analyses of mammalian ribonucleases have shown that gene duplication events giving rise to three paralogous genes occurred in ruminant ancestors. One of these genes encodes a ribonuclease identified in bovine brain. A peculiar feature of this enzyme and orthologous sequences in other ruminants are C-terminal extensions consisting of 17-27 amino acid residues. Evidence was obtained by Western blot analysis for the presence of brain-type ribonucleases in brain tissue not only of ox, but also of sheep, roe deer and chevrotain (Tragulus javanicus), a member of the earliest diverged taxon of the ruminants. The C-terminal extension of brain-type ribonuclease from giraffe deviates much in sequence from orthologues in other ruminants, due to a change of reading frame. However, the gene encodes a functional enzyme, which could be expressed in heterologous systems. The messenger RNA of bovine brain ribonuclease is not only expressed at a high level in brain tissue but also in lactating mammary gland. The enzyme was isolated and identified from this latter tissue, but was not present in bovine milk, although pancreatic ribonucleases A and B could be isolated from both sources. This suggests different ways of secretion of the two enzyme types, possibly related to structural differences. The sequence of the brain-type RNase from chevrotain suggests that the C-terminal extensions of ruminant brain-type ribonucleases originate from deletions in the ancestral DNA (including a region with stop codons), followed by insertion of a 5-8-fold repeated hexanucleotide sequence, coding for a proline-rich polypeptide. (C) 2001 Elsevier Science B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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