Effect of daunomycin on HeLa cell nucleic acid synthesis
| Autor: | A. Cohen, Laura E. Crook, K. R. Rees |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Electrophoresis
Cell Fractionation Tritium Biochemistry Cell Line HeLa chemistry.chemical_compound Leucine Protein biosynthesis RNA Messenger Uridine Pharmacology Messenger RNA Acrylamides Carbon Isotopes DNA synthesis biology Daunorubicin RNA DNA Ribosomal RNA biology.organism_classification Molecular biology chemistry RNA Ribosomal Depression Chemical Nucleic acid Half-Life HeLa Cells Thymidine |
| Zdroj: | Biochemical pharmacology. 21(3) |
| ISSN: | 0006-2952 |
| Popis: | In the presence of daunomycin (0.5 μg/ml) HeLa cell DNA synthesis was irreversibly inhibited. RNA synthesis although initially inhibited gradually reversed and returned to normal levels by 24 hr. Analysis showed that the drug selectively inhibited ribosomal RNA and only partially inhibited heterogeneous RNA which was altered in physical characteristics. Inhibition of protein synthesis was a late event indicating the continued synthesis of messenger RNA in the presence of daunomycin. A possible mechanism to explain these differential effects is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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