Improved Antithrombotic Function of Oriented Endothelial Cell Monolayer on Microgrooves
| Autor: | Jia-yan Chen, Bo-chao Li, Jian Ji, Yunbing Wang, Mi Hu, Ke-feng Ren, Hao Chang, He Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Materials science Flat surface Polydimethylsiloxane Biomedical Engineering 02 engineering and technology 021001 nanoscience & nanotechnology Biomaterials Endothelial stem cell 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Antithrombotic Monolayer 0210 nano-technology Vascular implant Cytoskeleton Function (biology) Biomedical engineering |
| Zdroj: | ACS biomaterials scienceengineering. 4(6) |
| ISSN: | 2373-9878 |
| Popis: | Achievement of an endothelial cell (EC) monolayer (re-endothelialization) on the vascular implant surface with competent and functioning features is critical for long-term safety after implantation. Oriented EC monolayer is beneficial to improve endothelial function such as enhanced athero-resistant property. However, the information about antithrombotic property of oriented EC monolayer is limited. Here, we used the microgrooved polydimethylsiloxane substrates to guide EC orientation and obtain oriented EC monolayer. The effects of anisotropic topography on EC behaviors and antithrombotic function of the EC monolayer were then evaluated. Our data demonstrated that ECs responded to grooves in a size-dependent way as shown in oriented cell cytoskeleton and nuclei, enhanced directed migration, and overall velocity. Furthermore, compared to the EC monolayer on the flat surface, the oriented EC monolayer formed on the grooved substrates exhibited improved antithrombotic capability as indicated by higher expression of functional related genes, production of prostacyclin and tissue plasminogen activator, and prolonged activated coagulation time. The improvement of antithrombotic function was especially notable on the smaller-size groove. These findings reveal the responses of ECs to varisized topography and antithrombotic function of the oriented EC monolayer, providing insights into optimal design of vascular implants. |
| Databáze: | OpenAIRE |
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