Determination of unsulfonated aromatic amines in FDC Yellow No. 6 by the diazotization and coupling procedure followed by reversed-phase high-performance liquid chromatography
| Autor: | Catherine J. Bailey, Naomi Richfield-Fratz, John E. Bailey |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
chemistry.chemical_classification
Chromatography Chloroform medicine.diagnostic_test Chemical Phenomena Organic Chemistry Extraction (chemistry) Salt (chemistry) General Medicine Diazonium Compounds Biochemistry High-performance liquid chromatography Benzidine Analytical Chemistry chemistry.chemical_compound Chemistry Aniline chemistry Spectrophotometry Phase (matter) medicine Spectrophotometry Ultraviolet Amines Azo Compounds Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. 331(1) |
| Popis: | Data are presented for the determination of parts-per-billion (10(9)) levels of aniline, benzidine, 4-aminobiphenyl (4-ABP) and 4-aminoazobenzene in the regulated color additive FD&C Yellow No. 6. The determination involves chloroform extraction of the amines from the color additive, followed by diazotization and coupling with the disodium salt of 3-hydroxy-2,7-naphthalenedisulfonic acid (R-salt). The coupling products are then analyzed by reversed-phase high-performance liquid chromatography. An interference discovered during the determination of 4-ABP required the use of 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylic acid (pyrazolone-T) as an alternative coupling agent. The identity of each coupling product is confirmed by obtaining a UV-visible spectrum of the eluting solute. The liquid chromatograph is calibrated in the presence of the color additive by using the external standard method. |
| Databáze: | OpenAIRE |
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