Circulation and genetic diversity of Feline coronavirus type I andII from clinically healthy andFIP ‐suspected cats in China
| Autor: | Dongbo Sun, Chunqiu Li, Donghua Guo, Fanzhi Kong, Qiujin Liu, Junjun Zhai, Mingjun Su |
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| Rok vydání: | 2018 |
| Předmět: |
Male
China medicine.medical_specialty Feline coronavirus 040301 veterinary sciences Biology medicine.disease_cause Feline Infectious Peritonitis law.invention 0403 veterinary science 03 medical and health sciences law Molecular genetics Genotype Prevalence medicine Animals Amino Acid Sequence Coronavirus Feline Genotyping feline coronavirus Phylogeny Feces Polymerase chain reaction 030304 developmental biology 0303 health sciences CATS General Veterinary General Immunology and Microbiology phylogenetic analysis S gene Genetic Variation Original Articles genetic diversity 04 agricultural and veterinary sciences General Medicine Virology Feline infectious peritonitis Spike Glycoprotein Coronavirus Cats Original Article Female Sequence Alignment |
| Zdroj: | Transboundary and Emerging Diseases |
| ISSN: | 1865-1682 1865-1674 |
| DOI: | 10.1111/tbed.13081 |
| Popis: | Feline infectious peritonitis (FIP) is a fatal infectious disease of wild and domestic cats, and the occurrence of FIP is frequently reported in China. To trace the evolution of type I and II feline coronavirus in China, 115 samples of ascetic fluid from FIP‐suspected cats and 54 fecal samples from clinically healthy cats were collected from veterinary hospitals in China. The presence of FCoV in the samples was detected by RT‐PCR targeting the 6b gene. The results revealed that a total of 126 (74.6%, 126/169) samples were positive for FCoV: 75.7% (87/115) of the FIP‐suspected samples were positive for FCoV, and 72.2% (39/54) of the clinically healthy samples were positive for FCoV. Of the 126 FCoV‐positive samples, 95 partial S genes were successfully sequenced. The partial S gene‐based genotyping indicated that type I FCoV and type II FCoV accounted for 95.8% (91/95) and 4.2% (4/95), respectively. The partial S gene‐based phylogenetic analyses showed that the 91 type I FCoV strains exhibited genetic diversity; the four type II FCoV strains exhibited a close relationship with type II FCoV strains from Taiwan. Three type I FCoV strains, HLJ/HRB/2016/10, HLJ/HRB/2016/11 and HLJ/HRB/2016/13, formed one potential new clade in the nearly complete genome‐based phylogenetic trees. Further analysis revealed that FCoV infection appeared to be significantly correlated with a multi‐cat environment (p |
| Databáze: | OpenAIRE |
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