Absence of microsatellite instability in breast carcinomas with both p53 and c-erbB-2 alterations
| Autor: | Cristina Formantici, Chiara Ronchini, Sylvie Ménard, Rosaria Orlandi, Silvana Pilotti, Maria I. Colnaghi, Guglielmina Nadia Ranzani |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Tumor suppressor gene Receptor ErbB-2 Breast Neoplasms Biology medicine.disease_cause Polymerase Chain Reaction Pathology and Forensic Medicine medicine Carcinoma Biomarkers Tumor Polymorphic Microsatellite Marker Humans skin and connective tissue diseases Microsatellite instability Middle Aged medicine.disease Phenotype Neoplasm Proteins Cancer research Microsatellite Female Tumor Suppressor Protein p53 Breast carcinoma Carcinogenesis Microsatellite Repeats |
| Zdroj: | The Journal of pathology. 187(4) |
| ISSN: | 0022-3417 |
| Popis: | Based on a previous finding that amplification of the c-erbB-2 oncogene and alteration of p53 are strongly associated in most aggressive breast tumours, the present study investigated whether microsatellite instability (MI) might also be associated with this tumour phenotype. Nine polymorphic microsatellite markers, including six dinucleotide, one trinucleotide, and two tetranucleotide repeats, were amplified from paired normal and tumour DNA samples of 15 breast tumours that overexpressed both c-erbB-2 and p53 and of 15 control breast tumours that overexpressed neither protein. All 30 breast tumours analysed exhibited a replication error-negative phenotype, with only one sample showing MI in one of the nine loci. This suggests that the genetic events underlying MI, which are critical in colorectal and gastric tumours, are not involved in the pathogenesis of c-erbB-2/p53 double-altered breast tumours and do not play a central role in breast tumour formation. |
| Databáze: | OpenAIRE |
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