Expression of carboxylesterase and lipase genes in rat liver cell-types
| Autor: | Alice Nakatsuka, William F. Bosron, Sonal P. Sanghani, Hidekazu Tsukamoto, Wilhelmina I. Davis, Tommaso Mello, Sharry L. Fears |
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| Rok vydání: | 2008 |
| Předmět: |
Kupffer Cells
Liver cytology Biophysics Gene Expression Cell Separation Polymerase Chain Reaction Biochemistry Article Carboxylesterase chemistry.chemical_compound Retinyl palmitate Animals Lipase Vitamin A Molecular Biology Cells Cultured Lipoprotein lipase biology Chemistry Endothelial Cells Cell Biology Rats Lipoprotein Lipase Liver Adipose triglyceride lipase Hepatocytes biology.protein Hepatic stellate cell Hepatic lipase Carboxylic Ester Hydrolases |
| Zdroj: | Biochemical and Biophysical Research Communications. 374:460-464 |
| ISSN: | 0006-291X |
| Popis: | Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells. As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation. |
| Databáze: | OpenAIRE |
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