A new method for studying the incorporation of nonesterified fatty acids into cardiac lipids by using deuterium-labelled palmitate
| Autor: | Hans Michael Piper, P. G. Spieckermann, J.F. Hütter, C. Schweickhardt, D.H. Hunneman |
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| Rok vydání: | 1988 |
| Předmět: |
Physiology
Palmitic Acid Palmitic Acids Fatty Acids Nonesterified 030204 cardiovascular system & hematology Gas Chromatography-Mass Spectrometry Glycerides Diglycerides Palmitic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine NEFA Physiology (medical) Animals Triglycerides 030304 developmental biology chemistry.chemical_classification 0303 health sciences Cholesterol Myocardium Albumin Fatty acid Rats Inbred Strains Lipid metabolism Metabolism Deuterium Lipid Metabolism Rats chemistry Biochemistry Cholesterol Esters Cardiology and Cardiovascular Medicine Perfusion |
| Zdroj: | Basic Research in Cardiology. 83:87-93 |
| ISSN: | 1435-1803 0300-8428 |
| DOI: | 10.1007/bf01907108 |
| Popis: | A new method for measuring the incorporation of exogenous fatty acids into myocardial lipids of working rat hearts using deuterium-labelled palmitate has been developed. After perfusing isolated hearts, the fatty acid composition of triglycerides, diglycerides, monoglycerides, cholesterol esters, and nonesterified fatty acids (NEFA) was measured by a mass fragmentographic method. Hearts perfused only with 5 mM glucose and 1 mM lactate perfusion as basic substrates showed a significant decrease of triglyceride content, while the other lipids were not found to be significantly reduced. The fatty acid composition of all lipids were not affected. An addition of D31-palmitate complexed to albumin at a molar ratio of 5:1, caused a dose-dependent incorporation into triglycerides and diglycerides which suggested saturation kinetics. The tissue content of nonesterified D31-palmitate was found to be linearly related to its concentration in the perfusate. It may be of note that the concentration of the other NEFA was significantly affected neither by a fatty-acid-free perfusion nor by a perfusion with D31-palmitate. A significant incorporation of the supplied fatty acid into cholesterol esters and monoglycerides could not be detected. |
| Databáze: | OpenAIRE |
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