Deoxynivalenol in wheat and maize flour reference materials 2. Preparation and certification
| Autor: | Peter J. Wagstaffe, S. Patel, John Gilbert, A. Boenke, Matthew Sharman |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Quality Control
Certification Chromatography Gas European community Health Toxicology and Mutagenesis Flour Food Contamination Toxicology Zea mays High-performance liquid chromatography chemistry.chemical_compound Drug Stability Vomitoxin Food science Mycotoxin Chromatography High Pressure Liquid Triticum Mathematics Chromatography Extraction (chemistry) Public Health Environmental and Occupational Health General Chemistry chemistry Chemistry (miscellaneous) Solvents Gas chromatography Laboratories Trichothecenes Food Analysis Food Science |
| Zdroj: | Food Additives and Contaminants. 9:119-135 |
| ISSN: | 0265-203X |
| DOI: | 10.1080/02652039209374055 |
| Popis: | The preparation of four cereal reference materials (two wheat and two maize) and the certification of their 4-deoxynivalenol (DON) contents is described. The materials were prepared and certified within the BCR programme of the Commission of the European Community as part of a broad activity to improve accuracy and agreement of measurements of importance in food and agriculture. Reference material RM 379 was prepared from naturally contaminated wheat four blended with 'blank' material to achieve the desired DON content. The reference material RM 378 was prepared from naturally contaminated maize identified as containing approximately the desired DON content. Details are given of the milling, blending and packaging procedure, and the checks to ensure homogeneity and stability of the materials. The reference materials RM 396 and 377 were similarly prepared from the blank wheat and maize samples respectively. The certification exercise was carried out by five laboratories (with a further two to three laboratories providing supporting data) using a variety of extraction and clean-up procedures with either high performance liquid chromatography (HPLC) or gas chromatography (GC) as the determinative stages. Wheat RM 379 was certified as containing DON at a level of 673 +/- 21 micrograms/kg and wheat RM 396 as containing less than 50 micrograms/kg. Maize RM 378 was certified as containing DON at 425 +/- 38 micrograms/kg and maize RM 377 as containing less than 50 micrograms/kg. The materials are intended for the verification of methods used to determine DON in cereal samples. |
| Databáze: | OpenAIRE |
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