Intracellular sodium in cardiomyocytes using 23Na nuclear magnetic resonance
| Autor: | Krzysztof Wroblewski, Haywood Blum, Mary Osbakken, Tamas Ivanics, Dah Jyuu Wang |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Iodoacetic acid Sodium chemistry.chemical_element Coronary Disease Iodoacetates Cell Separation chemistry.chemical_compound Nuclear magnetic resonance Extracellular Animals Myocyte Molecular Biology Chemistry Myocardium Cell Biology Buffer solution Iodoacetic Acid Rats Perfusion Reagent Quantitative analysis (chemistry) Mathematics Intracellular |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1221:133-144 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(94)90005-1 |
| Popis: | Intracellular sodium content in superfused isolated rat cardiomyocytes was measured using 23Na nuclear magnetic resonance. The shift reagent dysprosium tripolyphosphate was added to the buffer to distinguish between NMR signals from the intracellular region and the extracellular buffer. The NMR visibility of the intracellular sodium signal was experimentally determined by measuring the changes induced in the sodium NMR signals by application of ischemia as an intervention. Intracellular volume was accounted for by determining the change in the sodium signal upon adding cells (in beads) to the buffer solution at the beginning of each experiment and by killing the cells (in beads) with Triton X-100 at the end of each experiment. The visibility of intracellular sodium (relative to extracellular) was 0.47 +/- 0.12 (mean +/- S.D., n = 12). The average intracellular sodium concentration using this visibility is 29 +/- 4.5 mM (n = 12). This value is much higher than results obtained by some investigators using NMR techniques and by others using different standard methods, with the exception of those methods which evaluate the total intracellular sodium (atomic absorption spectroscopy and X-ray microanalysis). We conclude that total Nai is higher than generally reported, using other accepted techniques such as ion-specific electrodes, and that 23Na-NMR analysis can be used to accurately determine Nai in intact cells. |
| Databáze: | OpenAIRE |
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