Desensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen

Autor:	Martynas Smicius, Jiyoung Kim, Marie Christine Klitgaard, Lone Rønnov-Jessen, Nadine Goldhammer, Marc Baker Bechmann, Ole W. Petersen, René Villadsen
Rok vydání:	2021
Předmět:	S100A7 Cell biology medicine.drug_class Science Gene Expression Breast Neoplasms Diseases Mice SCID Stem cells S100 Calcium Binding Protein A7 Article Cell Line Andrology Breast cancer Endocrinology Downregulation and upregulation Cell Movement Mice Inbred NOD Pregnancy medicine Endocrine system Animals Humans Breast Cells Cultured Cell Proliferation Mice Knockout Multidisciplinary Estradiol business.industry Cell Differentiation Estrogens medicine.disease Cadherins Transplantation Receptors Estrogen Hormone receptor Estrogen Medicine Female business Receptors Progesterone Hormone
Zdroj:	Scientific Reports Rønnov-Jessen, L, Kim, J, Goldhammer, N, Klitgaard, M C, Smicius, M, Bechmann, M B, Villadsen, R & Petersen, O W 2021, ' Desensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen ', Scientific Reports, vol. 11, 17232 . https://doi.org/10.1038/s41598-021-96785-8 Scientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
ISSN:	2045-2322
DOI:	10.1038/s41598-021-96785-8
Popis:	Full term pregnancy at an early age is the only factor known to consistently protect against breast cancer. Because hormone receptor positive progenitors in the human breast relay endocrine signaling, we here sought to determine whether an experimental mimicry of the third trimester surge of hormones would change their susceptibility to growth stimulation. Hormone receptor positive, reduction mammoplasty-derived human breast epithelial progenitors were exposed to a short-term, pregnancy-level of estradiol, and their subsequent response to estradiol stimulation was analyzed. Exposure to pregnancy-level of estradiol results in subsequent lower sensitivity to estrogen-induced proliferation. Expression array and immunoblotting reveal upregulation of S100A7 and down-regulation of p27, both associated with parity and epithelial differentiation. Notably, we find that the epithelial differentiation is accompanied by upregulation of E-cadherin and down-regulation of vimentin as well as by diminished migration and more mature luminal epithelial differentiation in a mouse transplantation model. Our findings are in support of a de-sensitization mechanism for pregnancy-induced prevention against breast cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e69ee7c2ac27ac4b815fca0a5c9e1cd https://pubmed.ncbi.nlm.nih.gov/34446796 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.