The effect of partial vitrectomy on blood-ocular barrier function in the rabbit
| Autor: | Sally A. Scheib, William H. Garner, Bruce A. Berkowitz, Gustav Graff, Charles A. Wilson, Mizuto Suzuki |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Gadolinium DTPA
medicine.medical_specialty Blood-Aqueous Barrier genetic structures Ocular surgery medicine.medical_treatment Contrast Media Vitrectomy Vascular permeability Vascular leakage Retina Aqueous Humor Capillary Permeability Cellular and Molecular Neuroscience Magnetic imaging Ophthalmology Blood-Retinal Barrier Electroretinography medicine Animals Barrier function Treated group business.industry Retinal Vessels Blood–ocular barrier Magnetic Resonance Imaging eye diseases Sensory Systems Surgery Vitreous Body Rabbits sense organs business |
| Zdroj: | Current Eye Research. 23:372-381 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1076/ceyr.23.5.372.5439 |
| Popis: | Purpose. To compare ocular vascular permeability in the rabbit after vitrectomy as assessed by contrast-enhanced magnetic imaging (CE-MRI) and measurements of aqueous and vitreous humor protein concentration. Methods. Partial vitrectomies were performed, irrigating with BSS ® or BSS PLUS ® . Post-operative vascular leakage was determined by CE-MRI following intravenous administration of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA). Aqueous and vitreous protein concentrations were quantified by standard biochemical assay. ERG evaluations were performed on postoperative days 1, 3, and 7. Results. Using BSS as irrigant, breakdown of the inner blood-retinal barrier (BRB) occurred in 4/7 eyes on postoperative day 1. The rate of Gd-DTPA leakage was significantly greater on postoperative day 1 than that in unoperated, control eyes, but declined a50% by day 3. At both time points, outer BRB breakdown was restricted to the sclerotomy wounds. No BRB leakage was detectable in control eyes. Blood-aqueous barrier (BAB) leakage was bilateral on day 1. Significantly greater Gd-DTPA leakage occurred in the operated eye than in the nonsurgical contralateral eye. On day 3, a40% bilateral reduction in leakage indicated resolution of BAB leakage. Notably, Gd-DTPA leakage of the BAB and BRB was significantly reduced in the BSS PLUS treated group. In contrast to MRI assessments, protein concentrations of the aqueous and vitreous in the surgical eye showed no detectable differences between BSS and BSS PLUS. Concurrent with the transient loss of ocular barrier function, ERG responses also declined. However, by day 7 greater than 90% recovery was noted in BSS PLUS treated animals but not in the BSS treatment group. Conclusions. CE-MRI is capable of detecting subtle changes in vascular permeability following ocular surgery. Advantages of using BSS PLUS compared to BSS as the irrigating solution can be detected using this technique. BSS PLUS’s protection of barrier function is consistent with a rapid recovery in retinal function not observed in BSS treated eyes. |
| Databáze: | OpenAIRE |
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