Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing
| Autor: | Shue Jin, Yi Man, Li Liu, Chen Hu, Chenyu Chu, Renli Yang, Yili Qu, Chenbing Wang, Shengan Rung, Jidong Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Cell Biocompatible Materials Neogenesis 03 medical and health sciences Mice 0302 clinical medicine Immune system In vivo medicine Animals Research Articles 030304 developmental biology Skin 0303 health sciences Wound Healing Multidisciplinary biology integumentary system Tissue Scaffolds Chemistry Regeneration (biology) SciAdv r-articles biochemical phenomena metabolism and nutrition Hair follicle biology.organism_classification Cell biology Rats medicine.anatomical_structure Applied Sciences and Engineering 030220 oncology & carcinogenesis Wound healing Hair Follicle Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| Popis: | Scaffolds with aligned fibers promoted a T cell-dominant adaptive immune response in murine skin wound healing. The structural properties of biomaterials play crucial roles in guiding cell behavior and influencing immune responses against the material. We fabricated electrospun membranes with three types of surface topography (random, aligned, and latticed), introduced them to dorsal skin excisional wounds in mice and rats, and evaluated their effects on wound healing and immunomodulatory properties. An overview of different immune cells in the microenvironment with the help of single-cell RNA sequencing revealed diverse cellular heterogeneity in vivo. The time course of immune response was advanced toward an adaptive immunity–dominant stage by the aligned scaffold. In mice without mature T lymphocytes, lack of wound-induced hair neogenesis indicated a regulatory role of T cells on hair follicle regeneration. The microenvironment around scaffolds involved an intricate interplay of immune and cutaneous cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|