The carboxyl terminal sequence of nucleolar protein B23.1 is important in its DNA polymerase alpha-stimulatory activity
| Autor: | Keiji Sato, Yukio Furuichi, Shonen Yoshida, Mark O.J. Olson, Masaharu Takemura, Takao Takahashi, Satomi Usui, Hayato Umekawa, Yoshinori Watanabe |
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| Rok vydání: | 2001 |
| Předmět: |
HMG-box
DNA polymerase DNA polymerase II Molecular Sequence Data Biochemistry DNA polymerase delta Enzyme Stability Animals Protein Isoforms Amino Acid Sequence Molecular Biology Polymerase DNA clamp biology Chemistry Nuclear Proteins General Medicine Surface Plasmon Resonance DNA Polymerase I Molecular biology Recombinant Proteins Protein Structure Tertiary Rats Mutation biology.protein DNA polymerase I Primer (molecular biology) Nucleophosmin Protein Binding |
| Zdroj: | Journal of biochemistry. 130(2) |
| ISSN: | 0021-924X |
| Popis: | The protein B23 is a major nucleolar phosphoprotein comprising two isoforms, B23.1 and B23.2, which differ only in their carboxyl-terminal short sequences, the N-terminal 255 residues being identical in both forms. Both B23.1 and B23.2 stimulated immunoaffinity-purified calf thymus DNA polymerase alpha in a dose-dependent manner. The stimulatory effect of protein B23.1, the longer isoform, was found to be 2-fold greater than that of B23.2. Purified DNA polymerase alpha bound tightly to a protein B23.1-immobilized column, while it bound weakly to a protein B23.2-immobilized column. Surface plasmon resonance studies by BIAcore further showed that protein B23.1 bound to the DNA polymerase alpha-(dA).(dT) complex more tightly than did protein B23.2. The protein B23 isoforms appear to interact directly with the DNA polymerase alpha protein and not through the bound nucleic acid. These observations indicated that protein B23 physically bound to the DNA polymerase alpha and stimulated the enzyme activity. Product analyses showed that protein B23 greatly enhanced the reaction both in amount and length of product DNA, whereas it did not significantly alter the processivity of polymerization. In contrast, protein B23 effectively protected DNA polymerase alpha from heat inactivation. These results suggest that protein B23 stabilizes DNA polymerase alpha that is detached from product DNA, allowing the enzyme to be recruited for further elongation. Moreover, experiments using various C-terminal deletion mutants of protein B23 indicated that 12 amino acids at the C-terminal end of B23.1, which are absent in B23.2, may be essential for the full stimulation of the DNA polymerase alpha. |
| Databáze: | OpenAIRE |
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